2,4-D对大豆体细胞胚胎发生及增殖的影响

Somatic Embryogenesis and Proliferation of Soybean Embryogenic Tissue Treated with 2,4-D

研究不同浓度的2，4-D对不同基因型大豆体细胞胚胎发生及增殖的影响：结果表明：不同基因型大豆体细胞胚胎发生率存在显著差异，在供试的6种基因型中，以CH21141和黑农40体细胞胚胎发生率最高，黑农35诱导率最低；大豆体细胞胚胎发生及继代增殖的最佳2，4-D浓度为10～20mg·L^-1，继代扩繁系数为3^n（n为继代培养次数）.

Taking immature cotyledon of six soybean varieties as explants, the effect of 2,4- D on the frequencies of somatic embryogenesis and the proliferation of embryogenie tissue were investigated. Results showed that the frequencies of somatic embryogenesis varied with soybean genotypes, CH21141 and Heinong 40 had highest frequencies ,while Heinong 35 had the lowest frequency among six genotypes. The optimum media for somatic embryogenesis and the proliferation of embryogenie tissue was MS with 10-20mg·L^-1 2,4- D, and the proliferation speed of progeny was 3^n.