桑精胶囊对2型糖尿病大鼠抗氧化能力及Bax、Bcl-2蛋白表达的影响

Effect of Sangjing Capsule on Antioxidant Capacity and Bax and Bcl-2 Expression in Type 2 Diabetic Rats

目的探讨桑精胶囊治疗2型糖尿病的分子机制。方法采用尾静脉注射小剂量链脲佐菌素加高脂高热量饮食喂养的方法建立2型糖尿病大鼠模型,将造模动物随机分为模型组、二甲双胍组和桑精胶囊组,另设正常组对照。药物干预8周后,检测各组大鼠抗氧化能力指标超氧化物歧化酶(SOD)、丙二醛(MDA)、谷胱甘肽过氧化物酶(GSH-Px)、一氧化氮(NO)等,同时采用免疫组化法检测胰腺Bax、Bcl-2蛋白表达水平。结果模型组大鼠抗氧化能力及Bax、Bcl-2蛋白表达与正常组比较差异均有统计学意义(P<0.05或P<0.01);药物干预后,二甲双胍组、桑精胶囊组与模型组比较,SOD、GSH-Px、NO及Bax、Bcl-2蛋白表达均明显改善(P<0.05或P<0.01),其中对SOD、GSH-Px的改善桑精胶囊组要优于二甲双胍组(P<0.01)。结论桑精胶囊对2型糖尿病的治疗效应可能与其改善2型糖尿病大鼠氧化应激和抑制胰岛β细胞凋亡有关。

Objective To investigate the molecular mechanism of Sangjing Capsule （Mulberry capsule for diabetes） for type 2 diabetes. Methods The rat models of type 2 diabetes were established by intravenous injection of small dosage of streptozotocin plus high fat and high caloric diet. Then the animals were randomized into model group, metformin group and Sangjing Capsule group. A normal control group was set up for comparison. With medicinal intervention for eight weeks, the indices of antioxidant capacity including superoxide dismutase （SOD）, Malondialdehyde （MDA）, glutathion peroxidase （GSH-Px） and nitric oxide （NO） of all groups were examined, and the expression of Bax and Bcl-2 was tested with immunohistochemical method. Results In the model group, the an- tioxidant capacity and Bax and Bcl-2 expression were significantly different from those of the normal control group （P〈0. 05 or P〈0.01）. After medicinal intervention, in the metformin group and Sangjing Capsule group, SOD, GSH-Px, NO and expression of Bax and Bel-2 were all significantly improved as compared with the model group （P〈0. 05 or P〈0.01）, and SOD as well as GSH-Px was more significantly improved in Sangjing Capsule group than in metformin group （P〈0.01）. Conclusion The therapeutic effect of Sangjing Capsule on type 2 diabetes might be related to its ability of improving oxidative stress and inhibiting apoptosis of pancreas of rats.