维生素D受体基因多态性与汉族人肺结核发病的关系

Relationship between the genetic polymorphisms of VDR and susceptibility to pulmonary tuberculosis among the Chinese Han population

目的探讨维生素D受体(VDR)基因多态性与中国唐山地区汉族人肺结核发病的关系。方法采用病例对照研究,收集肺结核病例122例和健康对照者248例的环境因素暴露情况及静脉血,VDR基因多态性分析采用PCR-RFLP方法,利用TaqI、FokI和ApaI分析其基因多态性,以SPSS11.5软件对各危险因素进行单因素和多因素非条件Logistic回归分析。结果VDR-TT、VDR-Tt、VDR-FF、VDR-Ff、VDR-ff、VDR-AA、VDR-Aa、VDR-aa在病例组和对照组的频率分别为91.8%、8.2%、52.2%、28.3%、19.5%、51.2%、37.2%、11.6%和90.5%、8.5%、48.7%、31.1%、20.2%、53.3%、30.1%、15.6%,两组间差异无统计学意义(P均>0.05)。单因素和多因素分析均未发现VDR基因型与肺结核发病有关(P>0.05)。结论VDR基因型可能与汉族人肺结核发病无关。

Objective To explore the association between the genetic polymorphism of vitamin D receiver（VDR）and susceptibility to pulmonary tuberculosis in Chinese Han Population in Tangshan area.Methods The venous blood and environmental factors of 122 cases and 248 controls were collected.A case-control study was conducted.Polymorphism in VDR gene were detected by polymerase chain reaction with restriction fragment length polymorphism.The Fok I,Taq I and Apa I enzyme were employed.Univariate and multivariate logistic analyses were conducted with SPSS software package.Results The frequencies of genotype VDR-TT,VDR-Tt,VDR-FF,VDR-Ff,VDR-ff,VDR-AA,VDR-Aa,VDR-aa were 91.8%,8.2%,52.2%,28.3%,9.5%,51.2%,37.2%,11.6% and 90.5%,8.5%,48.7%,31.1%,20.2%,53.3%,30.1%,15.6% in cases and controls,respectively.The difference of genotype was not significant in cases and controls（all P〉0.05）.The univariate and multivariate logistic analyses demonstrated that the genetic polymorphisms of VDR on Fok I,Taq I and Apa I were not associated with susceptibility to pulmonary tuberculosis（P〉0.05）.Conclusion VDR genotypes might be not associated with the susceptibility to pulmonary tuberculosis among the Chinese Han Population.