供者淋巴细胞骨髓腔内输注对移植物抗宿主病和外周血IL-4与IFN-γ水平的影响

Influence of Intra-bone Marrow Infusion of Donor Lymphocytes on Development of Graft-Versus-Host Disease and Level of IL-4 and IFN-γ in Peripheral Blood

本研究观察骨髓腔内供者淋巴细胞输注对异基因小鼠外周造血干细胞移植(allo-PBSCT)后移植物抗宿主病(GVHD)和外周血IL-4和IFN-γ水平的影响。以雌性C57BL/6小鼠为受鼠,接受全身照射(TBI)预处理后,先骨髓腔移植雄性BABL/c小鼠来源的经rhG-CSF动员后的外周造血干细胞,然后分别经尾静脉和骨髓腔内进行供者淋巴细胞输注(DLI),建立异基因GVHD模型,观察移植后小鼠的生存状态和GVHD发生情况;以酶联免疫吸附实验(ELISA)检测白介素4(IL-4)和γ干扰素(IFN-γ)水平。结果显示:骨髓腔内-DLI组的受鼠GVHD发生比例和严重程度较尾静脉-DLI组明显减低(p<0.01);与尾静脉-DLI组比较,骨髓腔内-DLI组IL-4分泌增多,IFN-γ分泌减少(p<0.01)。结论:与尾静脉-DLI相比,IBM-DLI有利于减少GVHD的发生。

To investigate the effect of donor lymphocyte infusion （DLI） by intra-bone marrow （IBM） routes on the incidence of graft-versus-host disease（ GVHD）, level of IL-4 and IFN-γ after allogeneic peripheral hematopoietic stem cell transplantation （allo-PBSCT）. Female C57BL/6 mice as recipients received total body irradiation （TBI） on day 0, followed by injection of peripheral hematopoietic stem cells from mobilized donor of male BALB/c with the granulocytecolony stimulating factor （rhG-CSF）, and DLI was performed via ether IV or IBM routes. The severity of GVHD was compared in recipients received allogeneic IBM-DLI with those mice received IV-DLI; at 14 days after DLI, the levels of IL-4 and interferon （IFN）-3, were tested by ELISA. The results showed that as compared with IV-DLI group the frequency and severity of GVHD were reduced in IBM-DLI （p 〈 0.01 ） ; the level of IL-4 significantly increased, while the level of IFN-γ decreased in group IV-DLI （p 〈 0.01 ）. It is concluded that IBM-DLI declines the incidence and severity of GVHD after allo-PBSCT. Key words