期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

人Rb94基因联合γ射线照射对K150细胞生长影响的体外研究 被引量：5

Effects of human Rb94 gene transfer combined with γ-ray radiation on growth of esophageal carcinoma cells in vitro
原文传递
导出
摘要 目的探讨外源性Rb94基因联合γ射线照射对食管癌细胞株K150生长的抑制作用，为临床上进行砧94基因．放射治疗肿瘤奠定实验基础。方法将重组腺病毒Ad-Rb94于体外转染K150细胞，转染后进行6Gy^137Csγ射线照射，数字随机表法分为空白对照组、Ad-LacZ对照组、Ad—Rb94组、照射组和Ad-Rb94联合照射组，观察K150细胞的生长曲线、细胞周期、细胞凋亡和Rb94基因表达的变化。结果Ad-Rb94组、照射组和联合照射组对K150细胞生长均具有抑制作用，联合照射组的抑制效应最强（45．49％），明显高于Ad-Rb94组（20．26％）和照射组（35．11％）（χ^2=14．25，P〈0．05）。联合照射组K150细胞出现明显的G1期阻滞和细胞凋亡，G1期细胞和凋亡细胞所占比例最高，分别达到73．4％和15．7％。联合照射组K150细胞中Rb94基因表达量最多，分别是Ad—Rb94组和空白对照组的1.87倍和2．92倍。结论Rb94基因联合γ射线照射对食管癌细胞的抑瘤作用具有协同效应，有效地抑制肿瘤细胞的生长。 Objective To study the combined effect of foreign retinoblastoma 94 gene and ionizing radiation on the growth of esophageal carcinoma cells K150. Method K150 cells was transfected by recombinant Rb94 gene adenovirus vector and irradiated by 6 Gy 137 Cs 7-ray after transfection. The cohorts were divided into blank control, Ad-lacZ, Ad-Rb94, irradiation and Ad-Rb94 combined with irradiation. Cell growth, cell cycle, cell apoptosis and expression of retinoblastoma 94 gene of K150 cells were detected. Results The growth of K150 cells transfected with Ad-Rb94, radiation and Ad-Rb94 combined with irradiation group at 7 day after infection was all inhibited. The group of Ad-Rb94 combined with irradiation resulted in greater inhibition of cells growth, reached 45.49%, compared with Ad-Rb94 infection（20.26% ）and radiation group（35.11% ） （χ^2 = 14.25, P 〈 0.05）. Cells of G1 phases and apoptosis of K150 cells for the Ad-Rb94 combined with irradiation group were the highest, reached 73.4% and 15.7% , respectively. The combination of Ad-Rb94 and irradiation resulted in the greatest expression of retinoblastoma 94 gene, which was 1.87 fold and 2.92 fold of Ad-Rb94 infection and irradiation, respectively. Conclusions Ad-Rb94 gene transfeetion combined with in＇adiation shows the synergism for the inhibition of esophageal cancer K150 cells growth.
作者 王蕾 李进 姜恩海 宋力 刘强 张宁 唐卫生 王芹
机构地区 中国医学科学院放射医学研究所天津市分子核医学重点实验室
出处 《中华放射医学与防护杂志》 CAS CSCD 北大核心 2009年第6期568-570,589,共4页 Chinese Journal of Radiological Medicine and Protection
基金 国家自然科学基金（30770638）
关键词 RB94基因 食管癌细胞 照射 Retinoblastoma 94 gene Esophageal carcinoma Radiation
分类号 R73-3 [医药卫生—肿瘤]
  • 相关文献

参考文献11

  • 1Chakraborty S, Khare S, Dorairaj SK, et al. identification of genes associated with tumorigenesis of retinoblastoma by microarray analysis. Genomics, 2007,90(3) :344-353.
  • 2Li D, Day KV, Yu S, et al. The role of adenovirus-mediated retinoblastoma 94 in the treatment of head and neck cancer. Cancer Res, 2002,62 (16) : 4637-4644.
  • 3Zhang X, Multani AS, Zhon JH, et al. Adenoviral-mediated retinoblastoma 94 produces rapid telomere erosion, chromosomal crisis, and caspase-dependent apoptosls in bladder cancer and immortalized human urothelial cells but not in normal urothelial cells. Cancer Res, 2003, 63(4):760-765.
  • 4Roig JM, Molina MA, Cascante A, et al. Adenovirus-mediated retinobXastoma 94 gene transfer induces human pancreatic tumor regression in a mouse xenografl model. Clin Cancer Res, 2004, 10 (4) : 1456-1462.
  • 5赵艳芝,王芹,李进,穆传杰,唐卫生,王月英.人Rb94基因联合γ-辐射抑制乳腺癌细胞生长的研究[J].中国辐射卫生,2007,16(3):277-279. 被引量：8
  • 6Jain PT, Gewirtz DA. Sustained enhancement of liposome-mediated gene deliver and gene expression in human breast tumor cells by ionizing radiation. Int J Radiat Biol, 1999,75(2) : 217-223.
  • 7Teh BS, Aguilar-Cordova E, Vlachaki MT, et al. Combining radiotherapy with gene therapy (from the bench to the bedside ): a novel treatment strategy for prostate cancer. Oncologist, 2002,7 (5) : 458-466.
  • 8Kanazawa T, Mizukanfi H, Okada T, et al. Suicide gene therapy using AAV-HSVtk/ganeiclovir in combination with irradiation results in regression of human head and neck cancer xenografts in nude mice. Gene Ther, 2003,10( 1 ) :51-58.
  • 9Sun A, Bagella L, Tutton S, et al. From Go to S phase: A view of the roles played by the rctinoblastoma (Rb) family members in the Rb-E2F pathway. J Cell Biochem, 2007,102(6) : 1400-1404.
  • 10Araki K, Ahmad SM, Li G, et al. Retinoblastoma RB94 enhances radiation treatment of head and neck squamous cell carcinoma. Clin Cancer Res, 2008,14(11) : 3514-3519.

二级参考文献9

  • 1[1]LEE WEN-HWA,ROBERT BOOKSTEIN,FRANK HOMG,et al.Human retinoblastoma susceptibility gene:Cloning,Identification,and sequence[J].Science,1987,235:1 394-1 399.
  • 2[2]DAQING LI,KATHERINE V.DAY,SHUAHEN YU,et al.The role of adenovirus-mediated retinoblastoma 94 in the treatment of head and neck cancer[J].Cancer Research,2002,62:4 637-4 644.
  • 3[3]ZHANG XINQIAO,ASHA S MULTANI,JAIN-HUA ZHOU,et al.Adenoviral-mediated retinoblastoma 94 produces rapid telomere erosion,Chromosomal crisis,and caspase-dependent apoptosis in bladder cancer and immortalized human urothelial cells but not in normal urothelial cells[J].Cancer Research,2003,63:760-765.
  • 4[4]JOSEP MARIA ROIG,MIGUEL MOLINA,ANNA CASCANTE,et al.Adenovirus-mediated retinoblastoma 94 gene transfer induces human pancreatic tumor regression in a mouse xenograft model[J].Clinical Cancer Research,2004,10:1 456-1 460.
  • 5[5]STEVENS C W,ZENG M,CERNIGLIA G J,et al.Ionizing radiation greatly improves gene transfer efficiency in mammalian cells.Hum Gene Ther,1996,7(14):1 727-1 734.
  • 6[6]TANG D C,JENIGLIA R S,SHI Z,et al.Overexpression of adenovirus-encoded transgenes from the cytomegalovirus immediate early promoter in irradiated tumor cells.Hum Gene Ther,1997,8(17):2 117-2 124.
  • 7[7]KANAZAWA T,MIZUKAMI H,OKADA T,et al.Suicide gene therapy using AAV-HSVtk/ganciclovir in combination with irradiation results in regression of human head and neck cancer xenografts in nude mice.Gene Ther,2003,10(1):51-58.
  • 8[8]BADIE B,KRAMAR M H,LAU R,et a1.Adenovirus-mediated p53 gene delivery potentiates the radiation-induced growth inhibition of experimental brain tumors.J Neurooncol,1998,37(3):217-222.
  • 9[9]BIN S.THE,ESTUARDO AGUILAR-CORDOVA,MARLA T VLACHAKI,et al.Combining radiotherapy with gene therapy (from the bench to the bedside):A novel treatment strategy for prostate cancer,The Oncologist,2002,7:458-466.

共引文献7

同被引文献25

  • 1赵艳芝,李进,王芹,穆传杰.恶性肿瘤基因治疗和放射治疗相互作用的机制及联合治疗展望[J].国际放射医学核医学杂志,2006,30(4):250-253. 被引量：3
  • 2Li D,Day KV,Yu S,et al.The role of adenovirus-mediated retinoblastoma 94 in the treatment of head and neck cancer.Cancer Res,2002,62(16):4637-4644.
  • 3Zhang X,Multani AS,Zhou JH,et al.Adenoviral-mediated retinoblastoma 94 produces rapid telomere erosion,chromosomal crisis,and caspase-dependent apoptosis in bladder cancer and immortalized human urothelial cells but not in normal urothelial cells.Cancer Res,2003,63(4):760-765.
  • 4Roig JM,Molina MA,Cascante A,et al.Adenovirus-mediated retinoblastoma 94 gene transfer induces human pancreatic tumor regression in a mouse xenograft model.Clin Cancer Res,2004,10(4):1454-1462.
  • 5Parrish-Novak J,Dillon SR,Nelson A,et al. Interleukin 21 and its receptor are involved in NK cell expansion and regulation of lymphocyte function. Nature,2000,408(6808):57-63.
  • 6Monteleone G, Sarra M, Pallone F. Interleukin-21 in T cell-mediated diseases. Discov Med,2009,8(42):113-117.
  • 7Sondergaard H, Skak K. IL-21: roles in immunopathology and cancer therapy. Tissue Antigens,2009,74(6):467-479.
  • 8Keall PJ, Lammering G, Lin PS,et al. Tumor control probability prediction for genetic radiotherapy. Int J Radiat Oncol Biol Phys,2003,57(1):255-263.
  • 9Sun A, Bagella L, Tutton S, et al. From G0 to S phase: a view of the roles played by the retinoblastoma (Rb) family members in the Rb-E2F pathway. J Cell Biochem, 2007,102(6):1400-1404.
  • 10Araki K, Aamad SM. Retinoblastoma RB94 enhances radiation treatment of head and neck squamous cell carcinoma. Clin Cancer Res,2008,14(11):3514-3519.

引证文献5

二级引证文献5

中华放射医学与防护杂志
2009年 第6期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部