血清超敏C-反应蛋白水平及CRP 1059G/C基因多态性与脑梗死的相关性

Relationship between the serum high-sensitivity C-reactive protein level,C-reactive protein 1059G/C gene polymorphism and cerebral infarction

目的探讨超敏C-反应蛋白(hs-CRP)水平及CRP1059G/C基因多态性与脑梗死(CI)的相关性。方法采用多聚酶链式反应和限制性片段长度多态性(PCR-RFLP)方法检测105例CI患者和121名对照者CRP1059G/C基因型和等位基因频率;应用免疫透射比浊法测定血清hs-CRP水平。分析CI患者病情与血清hs-CRP水平及CRP1059G/C基因型的关系。结果CI组CRP1059G/G基因型和G等位基因频率显著高于对照组,G/C+C/C基因型和C等位基因显著低于对照组(均P<0.05);CRP1059G/G、G/C、C/C基因型血清hs-CRP水平依次降低;重度CI患者血清hs-CRP水平显著高于轻、中度组(均P<0.05),但CRP1059G/C基因型和等位基因频率与CI病情无关。回归分析显示血清hs-CRP水平是CI的危险因素之一;G/C+C/C基因型与降低CI的发病相关。结论CRP1059G/C基因型与血清hs-CRP水平相关;血清hs-CRP水平与CI病情相关;CRP1059基因中C等位基因可能是汉族CI的保护因素。

Objective To explore the relationship between the level of high-sensitivity C-reactive protein （ hs- CRP）, CRP 1059G/C gene polymorphism and cerebral infarction（CI）. Methods The CRP 1059G/C genotype and allele frequencies were assayed by polymerase chain reaction-restricted fragments length polymorphism （PCR-RFLP） in 105 patients with CI and 121 controls. The level of serum hs-CRP was detected by immune-turbidimetry. The relationship between the condition of CI patients, the level of serum hs-CRP and CRP 1059G/C genotype and allele frequencies were anaysed. Results Compared to the control group, the CRP 1059G/G genotype and G allele frequencies in CI group were statistically higher, G/C ＋ C/C genotypes and C allele frequencies were statistically lower（ all P 〈 0.05 ）. The levels of serum hs-CRP with CRP 1059G/G, G/C, C/C genotypes in CI group were decreased systematically. The level of serum hs-CRP in severe CI patients was significantly higher than those in lighter and medium CI patients （ all P 〈 0. 05）. However, there was no significant association between the CRP genotype and alleles frequencies and CI patient＇ s condition. The regression analysis showed that the level of serum hs-CRP was one of the CI risk factors. The CRP 1059 G/C ＋ C/C genotypes were related to decreased the CI occurrence. Conclusions CRP 1059G/C genotype is related to the serum hs-CRP level. The serum hs-CRP level is related to the condition of CI patient. The C allele of CRP 1059 may be a protective factor of CI in Chines.