糖皮质激素受体基因第4内含子G1666T多态性与脑出血的相关性

Correlation between the gene polymorphism of glucocorticoid receptor intron 4 G1666T and intracerebral hemorrhage

目的探讨糖皮质激素受体(GR)基因第4内含子G1666T(GRG1666T)多态性与脑出血(ICH)的相关性。方法应用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)方法检测48例ICH患者(ICH组)、48例ICH伴高血压(EH)患者(ICH+EH组)、46例单纯高血压患者(EH组)及71名健康对照者(NC组)GRG1666T基因型和等位基因频率;比较不同基因型ICH患者的性别、体质量指数(BMI)、血压、血管紧张素原、肾素、血糖、血脂水平的差异。结果4组间GRG1666T的基因型和等位基因频率差异无统计学意义;ICH组、ICH+EH组及EH组女性患者G等位基因频率明显高于NC组(均P<0.05)。GG、GT、TT基因型女性ICH组患者的BMI、血管紧张素原水平和血压依次显著降低(均P<0.05);GG基因型ICH组患者中,女性上述指标明显高于男性(均P<0.05)。结论GRG1666TG等位基因可能是中国女性ICH的易感基因之一。

Objective To explore the correlation between the gene polymorphism of glucocorticoid receptor intron 4 G1666T （GR GI666T） and intracerebral hemorrhage （ICH）. Methods Frequencies of genotypes and alleles of GR G1666T in 48 pations with ICH（ ICH group）, 48 ICH patients combining essential hypertension（EH） （ICH ＋ EH group）,46 EH patients （EH group） and 71 healthy people （NC group ） were analyzed by a polymerase chain reaction for restriction fragment length polymorphism （PCR-RFLP）. The gender, body mass index （ BMI ） , biood pressure（BP） , the levels of plasma angiotensinogen, renin, blood glucoseand and blood lipid were compared among the various genotype of GR G1666T in ICH patients. Results There was no obviously difference of G1666T genotype and allele frequencie among the 4 groups. However, the G allele frequence of females in groups ICH, ICH ＋ EH and EH were significantly higher than that in NC group（ all P 〈 0.05）. In the ICH patients, the BMI, BP and the levels of plasma angiotensinogen of female with GG, GT, TT genotypes were remarkable decreased systematically（ all P 〈0. 05 ）. In the ICH patients with GG genotype ,those indexes in female were significantly higher than in male（ all P 〈 0. 05 ）. Conclusion The G allele of GR G1666T in Chinese female may be one of the ICH susceptibility genes.