聚肌胞刺激后慢性乙型肝炎患者树突状细胞内Toll样受体3的表达变化

Expression of Toll-like receptor 3 on the peripheral blood dendritic cells after stimulated by poly I： C in patients with chronic hepatitis B

目的研究慢性乙型肝炎（CHB）患者树突状细胞（DC）内Toll样受体3（TLR3）的表达变化，探讨HBV持续感染与TLR3表达之间的关系。方法选取CHB患者60例（CHB组）及健康对照者20名（对照组）为研究对象，应用免疫磁珠细胞分选法获得CD14^＋单核细胞，体外诱导培养成未成熟的髓样树突状细胞（imDC），并以聚肌胞（polyI：C）刺激以获取成熟的树突状细胞（mDC）。刺激48h后测定细胞内TLR3蛋白及mRNA的表达，表面标志CD80和人白细胞抗原（HLA）-DR的蛋白表达。计量资料采用t检验，计数资料采用卡方检验。结果poly I：C刺激前，对照组和CHB组imDC内TLR3的流式平均荧光密度（MFI）分别为1192．95±301．40和1212．05±250．80，组间差异无统计学意义（t=0．280，P〉0．05）；刺激后MFI均明显升高，对照组与CHB组分别为1593．00±349．65和1352．98±313．67，前者比后者升高更显著（t=2．880，P〈0．05）。poly I：C刺激后，对照组与CHB组mDC胞内TLR3mRNA的表达水平均明显升高，分别为0．1780±0．0664和0．1204±0．0267，前者升高更显著（t=3．909，P〈0．05）。CHB组内再分为HBeAg（＋）和HBeAg（-）组，刺激后两组mDC内TLR3的MFI和mRNA水平均较imDC有明显升高，但组间比较差异均无统计学意义（t=0．366，P〉0．05）。结论polyI：C刺激后，对照组和CHB组中mDC胞内TLR3的表达均明显升高，但CHB患者升高的程度显著低于健康者，提示CHB患者的TLR3合成不足可能与HBV持续感染相关。

Objective To elucidate the expression of Toll like receptor 3 （TLR3） on dendritic cells（DCs） in patients with chronic hepatitis B （CHB）, and to explore the correlation between hepatitis B virus （HBV） persistent infection and TLR3 expression. Methods Sixty CHB patients （CHB group） and 20 healthy controls （control group） were enrolled. The peripheral blood mononuclear cells （PBMCs） were isolated and CD14^＋ monocytes were sorted by immunomagnetic beads. Immature DCs （imDC） were induced and proliferated in vitro and mature DCs （mDC） were obtained after the poly I： C stimulation. The expression of intracellular TLR3 mRNA was detected by real-time polymerase chain reaction （PCR）, and surface markers ECD80 and human leucocyte antigen （HLA）-DR] were determined by flow cytometry after 48 h of stimulation. The comparison of quantitative data was done using t test. The qualitative data were compared using cbi-square test. Results The mean fluorescence intensities （MFI） of intracellular TLR3 of imDC before poly I：C stimulation in CHB group and control group were 1212.05±250.80 and 1192.95 ± 301.40, respectively, which were not significantly different （t = 0. 280, P〉0.05）. While after stimulation, those were 1352.98±313.67 and 1593.00±349.65, respectively, the latter was significantly higher than the former （t=2. 880, P〈0.05）. The levels of TLR3 mRNA inside mDCs in both groups were increased after poly I：C stimulation, which were 0. 1204±0. 0267 and 0. 1780±0. 0664, respectively in CHB group and control group, and that in control group was significantly higher （t= 3. 909, P〈 0.05）. Furtherly, patients in CHB group were divided into HBeAg（＋） and HBeAg（-） subgroups. After stimulation, the MFI and mRNA of TLR3 inside mDC were greatly elevated in both subgroups, but there were no difference between these two subgroups （t = 0. 366, P〉0. 05）. Conclusions The intraeellular expressions of TLR3 in mDC in CHB group and control group are obviously increased aher the poly I： C stimulation, but the increased level in CHB group is lower than that in control group. The results suggest that the insufficiency of TLR3 synthesis may be related to the HBV persistent infection.