慢性乙型肝炎患者B7-H1和PD-1信号途径表达的特点

The expressions of B7-H1 and PD-1 signal pathway in patients with chronic hepatitis B

目的初步探讨免疫细胞协同共刺激分子途径之一——B7-H1和PD-1表达在慢性乙型肝炎（CHB）患者中的特点。方法收集11例CHB患者和16例健康人的外周血肝素抗凝标本，流式细胞技术检测CHB患者外周血髓样树突细胞（mDC）和T淋巴细胞上B7-H1和PD-1的表达。数据比较采用t检验。结果CHB患者外周血CD3^＋T淋巴细胞、CD4^＋T淋巴细胞、CD8^＋T淋巴细胞和mDC上B7-H1阳性表达率分别为（40．69±14．49）％、（42．84±11．19）％、（33．48±14．07）％和（16．60±4．04）％，健康对照者分别为（14．664±10．11）％、（4．62±3．84）％、（1．89±2．31）％和（0．49±0．37）％，CHB患者B7-H1在T淋巴细胞和mDC上的表达水平均明显高于健康者，差异具有统计学意义（t=-2．884。t=-10．894，t=-7．378，t=-13．182；均P〈0．05）。CHB患者CD3^＋T淋巴细胞、CD4^＋T淋巴细胞和CD8^＋T淋巴细胞上PD-1阳性表达率分别为（12．45±6．36）％、（11．42±6．20）％和（13．03±6．71）％，健康对照者分别为（7．80±3．53）%、（7．12±2．60）％和（7．88±3．74）％，CHB患者PD-1在T淋巴细胞上的表达水平也均明显高于健康者，差异具有统计学意义（t=-2．323，t=-2．355，t=-2．439；均P〈0．05）。结论B7-H1和PD-1在CHB患者外周血mDC和T淋巴细胞上表达较高。

Objective To study the expressions of B7-H1 and PD-1 which are costimulatory molecules in the pathogenesis of chronic hepatitis B （CHB）. Methods The heparin anticoagulant blood samples from 11 CHD patients and 16 healthy controls were collected. The expressions of BT-H1 and PD-1 on myloid dendritic cells （mDC） and T lymphocytes in the peripheral blood were analyzed by flow cytometry. The data comparison was done by t test. Results The expressions of B7-H1 on CD3^＋ , CD4^＋ , CD8 ^＋ T lymphocytes and mDC in CHB patients were （40.69 ± 14.49） %, （42.84 ± 11. 19 ） %, （33.48± 14.07） % and （16. 604±4.04） % , respectively, and those were （14.66±10.11）% , （4.62± 8.84） %, （1.89±2.31）% and （0.49±0.37）% , respectively in healthy controls. The expressions of BT-H1 on T-lymphocytes and mDC in CHB patients were significantly higher than those in healthy controls, and the differences were significant （t=-2. 884, t=- 10. 894, t=- 7. 378, t=-13. 182, respectively; all P〈0.05）. The expressions of PD-1 on CD3^＋ , CD4^＋ and CD8^＋ T lymphocytes in CHBpatients were （12.45±6. 36）% , （11. 42±6. 20）% and （13. 03±6.71）%, respectively, and those were （7.80±3.53）% , （7.12±2.60） % and （7.88±3.74） %, respectively in healthy controls. The expressions of PD-1 on T lymphocytes in CHB patients were significantly higher than those of the healthy controls, the differences were significant （t =-2.323, t =- 2. 355, t =- 2. 439, respectively; all P〈0.05）. Conclusion The expressions of B7 H1 and PD-1 on T lymphocytes and mDC in CHB patients are higher than the healthy people.