生长激素对化疗大鼠机体状态和骨髓功能的影响

Effect of Human Growth Hormone on Growth Situation and Hematopoietic Function in Rats under Chemotherapy

目的探讨人生长激素(hGH)对化疗大鼠机体状态和造血功能的影响。方法实验选用健康成年SD大鼠136只,体重(200±50)g,标准喂养,采用随机数字表法随机分为5组:正常对照组(空白组,n=8)、生理盐水对照组(NS组,n=32)、生长激素组(hGH组,n=32)、化疗组(CT组,n=32)及CT+hGH组(n=32)。分别于实验前及给药后第7、14、21及28天称量大鼠体重,采用骨髓涂片、病理切片及免疫组化法(增殖细胞核抗原检测)检测大鼠骨髓造血功能。结果给药后第7天CT+hGH组大鼠体重下降程度较CT组明显减轻(P<0.05);CT组和CT+hGH组骨髓增生极度低下或明显减低(P<0.05)。第14天CT+hGH组大鼠体重已为正增长,而CT组仍表现为体重丢失;CT+hGH组骨髓增生极度活跃,骨髓有核细胞总数较CT组明显增加(P<0.05)。第7、14及21天CT组增殖细胞核抗原阳性细胞数均明显低于hGH组和CT+hGH组(P<0.05)。给药后第7天hGH组大鼠体重较空白组明显增加(P<0.05),其他不同时间点空白组、NS组与hGH组各指标检测结果差异均无统计学意义(P>0.05)。结论hGH能减少化疗大鼠的体重丢失,对化疗大鼠的骨髓抑制有一定的保护作用。

Objective To investigate the effect of recombinant human growth hormone （hGH） on growth situation and bone marrow hematopoietic function in rats under chemotherapy.Methods A total of 136 10-week-old SD rats were included in the study.The rats were randomly assigned into five groups：normal control group （n=8）,normal saline control group （NS group,n=32）,human growth hormone group （hGH group,n=32）,chemotherapeutic drug treated group （CT group,n=32）,and chemotherapeutic drug plus hGH treated group （CT＋hGH group, n=32）. The body weight, bone marrow differential count, and the expression of proliferating cell nuclear antigen （PCNA） in bone marrow were measured before treatment and weekly in four weeks after treatment. Results Weight loss occurred in both CT group and CT＋hGH group （P〈0.05）, but the weight loss in CT＋hGH group was significantly smaller （P〈0.05） on day 7 after treatment. In myeloid morphology, myeloid cell was hypoplastic excessively in CT group, and it was hypoplaslic obviously in CT＋hGH group on day 7 after treatment. Day 14, weight gain appeared in CT＋hGH group, while weight loss remained in CT group; In myeloid morphology, myetold cell was hyperplastic actively excessively in CT-FhGH group, and myeloid karyote count was increased significantly in CT＋hGH group （P〈0.05）. Day 7, 14 and 21, PCNA positive cells count in CT group was lower than that in hGH group and CT＋hGH group （P〈0.05）. There was no significant different of every index among normal control group, NS group, and hGH group （P〉0.05）, except weight between normal control group and hGH group on day 7 （P〈0.05）. Conclusion hGH has a protective effect on myeloid hematopoietic function and growth situa- tion in rats after intraperitoneal chemotherapy.