小剂量希罗达抗小鼠结肠癌生长及微血管的形成

Antiangiogneic Effect and Antitumor Effect of Minidose Xeloda on Mice with CT-26 Coloretical Carcinoma

目的研究小剂量希罗达对小鼠结肠癌CT-26移植瘤生长和微血管生成的影响。方法建立小鼠结肠癌皮下移植瘤模型,随机分组:治疗组持续小剂量希罗达90mg/kg(每只0.1ml)灌胃,对照组给予相应体积生理盐水。给药后观察每组小鼠肿瘤生长情况,肿瘤长出后隔天测量肿瘤体积,接种肿瘤细胞两周后,处死全部小鼠,免疫组织化学法检测肿瘤组织血管内皮生长因子(VEGF)表达及肿瘤组织微血管密度(MVD),Western blot法检测肿瘤组织VEGF的表达。结果治疗组肿瘤生长受到明显抑制,治疗组VEGF表达强度及MVD低于对照组,VEGF与MVD间呈正相关;Western blot检测治疗组VEGF表达明显减弱。实验结束时未见明显不良反应。结论小剂量希罗达能通过抑制肿瘤微血管生成发挥抗肿瘤作用,其机制与抑制VEGF的表达有关。

Objective To evaluate the antiangiogenic effect and antitumor effect of the minidose Xeloda on the mice with coloretical carcinoma.Methods Sixteen Balbc mice modle with CT-26 coloretical carcinoma were established and randomized into two groups：Tested group （continues low dose Xeloda,90 mg/kg） and control group（0.9% sodium chloride）.Then the size of tumor、weight of mice were observed.At the end of the experiment（2 weeks after modle making ）,tumor tissue was given immunohistochemical staining. Tumor microvascular density （MVD） and vascular endothelial growth factor （VEGF） expression were detected, and VEGF expression was detected by Western blot. Results Compared with control group, tumor growth of tested group was obviously restrained. MVD and VEGF expression were decreased in tested group. There was no toxic reaction in tested group. Conclusion The continuous low dose of Xeloda played a role of antitumor by holding tumor vascularization. The possible mechanism is inhibiting the expression of VEGF.