艾迪注射液对裸鼠人胃癌移植瘤的抑瘤作用研究

Inhibitory Effects of Aidi Injection on Transplanted Human Gastric Cancer in Nude Mice

目的:观察艾迪注射液对裸鼠人胃癌移植瘤的抑瘤作用。方法:将人胃癌细胞BGC-823接种于裸鼠右腋皮下,建立荷瘤裸鼠模型;将荷瘤裸鼠随机分为5组:模型组、环磷酰胺组和艾迪注射液高、中、低剂量组,各组动物按相应药物和剂量给药,连续14d;末次给药后处死动物,剥取瘤块,称重计算抑瘤率并作组织学观察。结果:艾迪注射液高剂量组的抑瘤率为61.84%,光镜下可见大片状的坏死区。结论:艾迪注射液对裸鼠人胃癌细胞BGC-823具有抑制作用。

Objective：To observe the anti-cancer effects of Aidi injection（Ad）.Methods：Gastric cancer model of nude mice were established by transplanting human gastric cancer cell line（BGC-823）into hypoderm at mouse right armpit,and then the cancer-bearing mice were divided into 5 groups：model group,cyclophosphamide（CTX）group,and 3 Ad treated groups（high-dose,middle-dose,and low-dose groups）.Physiologic saline,CTX,Ad were given to peritoneal cavity of mice of different groups respectively for 14 days.The mice were sacrificed by decapitation,and tumor tissues were weighted and observed with histological technique afterwards.Tumor growth inhibitory rates were calculated.Results：The tumor weighing showed that after administration,tumor growth in high-dose Ad group was apparently slower,with an inhibitory rate of 61.84%（P0.05）.Histochemistry analysis indicated the transplanted tumor was poorly differentiated gastric cancer,and necrosis could be observed within tumor tissues,especially in CTX and Ad treated groups.Conclusion：Ad has inhibitory effect on transplanted human gastric cancer in nude mice.