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金鱼β-catenin以细胞自主性方式抑制神经发育早期调节基因vsx1的表达

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摘要 β-catenin基因是脊椎动物背部中轴结构形成的必需基因.近年的研究发现,在斑马鱼和爪蟾中,β-catenin还具有抑制神经外胚层形成的作用.为深入了解β-catenin是如何抑制神经外胚层形成以及这种抑制在正常发育过程中的功能,我们研究了金鱼胚胎发育过程中β-catenin对神经外胚层发育早期调节基因vsx1表达的抑制作用.实验结果表明,用反义morpholino oligonucleotides(MO)抑制内源β-catenin的功能可导致胚胎发育早期vsx1的广泛表达;β-catenin可抑制vsx1基因启动子所控制的绿色荧光蛋白(GFP)报告基因的表达.进一步的分析证明,β-catenin所直接启动的下游靶基因boz可以通过vsx1基因启动子中的特定结合位点抑制vsx1基因启动子所控制的GFP报告基因的表达.这些结果表明,β-catenin在脊索中胚层前体细胞中启动与脊索中胚层发育调节相关基因表达的同时,还能以细胞自主性方式抑制神经发育早期调节基因vsx1在这些细胞中表达,提示β-catenin在脊索中胚层前体细胞中抑制vsx1基因的异位表达与启动脊索中胚层调节基因的表达都是保障脊索正常发育所必需的.
作者 张琼宇 郑康 马珊珊 童英 罗琛
机构地区 湖南师范大学生命科学学院 浙江大学生命科学学院
出处 《科学通报》 EI CAS CSCD 北大核心 2009年第22期3455-3463,共9页 Chinese Science Bulletin
基金 国家自然科学基金重点项目(批准号:30430370) 国家重点基础研究发展计划项目(编号:2004CB117401)资助
关键词 Β-CATENIN VSX1 boz 脊索中胚层 发育
分类号 Q3 [生物学—遗传学]
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参考文献31

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