硫代乙酰胺诱导大鼠肝纤维化模型的制备

Establishment of Rat Hepatic Fibrosis Model Induced by Thioacetamide

目的:建立稳定高效的硫代乙酰胺(thioacetamide,TAA)大鼠肝纤维化模型,分析大鼠肝脏病理纤维化分级与血清检测物指标,为临床提供适用的监测手段及理论依据。方法:选取SD雄性大鼠48只,随机分为4组,即对照组(12只)、模型-Ⅰ组(12只)、模型-Ⅱ组(12只)和模型-Ⅲ组(12只)。根据大鼠体重,采用腹腔注射TAA诱导肝纤维化。按实验给定时间给药,在不同时间段内测定谷草转氨酶(AST)和谷丙转氨酶(ALT)血清浓度,并进行肝脏病理组织学检查。结果:模型-Ⅰ组大鼠死亡率为25.00%(3/12)、肝纤维化形成率为75.00%(9/12);模型-Ⅱ组大鼠死亡率为8.33%(1/12)、肝纤维化形成率为91.67%(11/12);模型-Ⅲ组肝纤维化形成率为83.33%(10/12)。各组AST和ALT血清浓度均升高,肝脏病理组织学检查发现肝组织病变。结论:本法病理组织学检查结果可靠,操作简便,适用于实验肝纤维化模型制备。

Objective： To provide suitable clinical monitoring method and theory by establishing the stable and efficient rat hepatic fibrosis model under the induction of thioacetamide （TAA）, and by analyzing hepatic pathology classification and serum test index of rat .Methods： 48 SD male rats were selected and randomly divided into 4 groups, i.e. control group （12）, model- Ⅰ group（12）, model-Ⅱ group（12） and model-Ⅲ group（12）. According to the rat weight, using TAA intmperitoneal injection to induce hepatic fibrosis model. Drugs were injected according to the time of experiment, test serum concentrations of AST and ALT in different time periods and the histopathology examination of hepar was conducted. Results： Mortality of model - Ⅰ was 25.00% （3/12）, the rate of hepatic fibrosis is 75.00%（9/12）; mortality of model - Ⅱ is 8.33%（1/12） and the rote of hepatic fibrosis was 91.67% （11/12）; the rate of hepatic fibrosis of model - Ⅲ was 83.33%（10/12）. Serum concentrations of AST and ALT increased in all group, and the liver lesions were found in liver pathological and histological examination. Conclusion： This histopathology result is reliable and simple to operate, and it is suitable for preparation of experimental hepatic fibrosis model .