HPLC法测定咳可合剂中绿原酸的质量浓度

Determination of Chlorgenic Acid in Keke Mixture by HPLC

目的：建立高效液相色谱法（HPLC）测定咳可合剂中绿原酸质量浓度的方法。方法：采用C18色谱枉，以乙腈-0．2％磷酸（8：92）为流动相，流速为1．0mL／min，检测波长为327nm，柱温为室温。结果：绿原酸在质量浓度36．30-363．00μg/mL范围内与峰面积响应值线性关系良好，r=0．9998。样品溶液在24h内稳定，平均加样回收率为99．93％，RSD=1．25％（n=5）。结论：本法操作简便，结果准确可靠．可作为该制剂的定量分析方法。

Objective： To establish the determination method of chlorgenic acid in Keke mixture by HPLC. Methods： The separation was performed on C18 column with acetonitrile-0.2% phosphoric acid （8 ： 92） as mobile phase and 1.0 mL/min of the flow rate, the detection wavelength was at 327 nm, and the colunm temperature was room temperature. Results： The linear range of chlorlgenic acid was in the range of 36.30-363.00 μg/mL, r = 0.999 8. The average recovery was 99.93 %, and RSD was 1.25 %（n = 5）. Conclusion： The method was rapid, sensitive and accurate. It can be used for quality control of Keke mixture.