摘要
[目的]研究参芪复方对GK大鼠心肌细胞凋亡相关因子Bcl-2、Bax及一氧化氮(NO)的影响及其可能作用机制。[方法]GK大鼠腹腔注射N-硝基-L-精氨酸甲酯(L-NAME),同时给予高脂饮食复制2型糖尿病(T2DM)心肌病变模型。选择随机血糖≥11.1mmol/L的GK大鼠随机分为4组:模型组、中药高、低剂量组、西药组,另设正常Wistar大鼠作正常对照组,共5组动物。治疗4周后,统一取心肌标本,通过免疫组化等测量方法,对照观察各组药物对GK大鼠一般状态、心肌NO、心肌Bcl-2、Bax蛋白表达的影响。[结果]GK大鼠的上述指标存在明显异常。参芪复方特别是其高剂量组能改善其一般状态、升高心肌NO(P<0.01),升高心肌细胞凋亡抑制因子Bcl-2的表达(P<0.01),降低促细胞凋亡因子Bax表达(P<0.05)和Bax/Bcl-2比值(P<0.01)。[结论]凋亡相关因子Bcl-2和Bax与NO参与糖尿病心肌损伤过程,参芪复方能升高心肌NO,降低心肌Bax表达,升高Bcl-2表达。
[Objective] To research the effective mechanisms of ShenQi Compound Prescription having action on myocardial cell Bcl-2, Bax and NO in GK rats (Goto-Kakizaki wistar rats) of T2DM. [Methods] The DCM was induced in rats by i.p. injecting Nω-nitio-L-arginine methyl ester (L-NAME) and gavaging high fat diet. The GK rats (RBS≥11.1 mmol/L)were divided into four groups: model control group, ShenQi Compound Prescription high-dose group, ShenQi Compound Prescription low-dose group, control group, at the same time cure group treated by ShenQi Compound Prescription for four weeks to observe the common state, the levels of NO of myocardium, Bcl-2 and Bax proteinum expression.[Results] ShenQi Compound Prescription could ameliorate GK rats’ common state, increase NO of myocardium, increase the expression of Bcl-2 protein, inhibit the Bax protein expression, adjust Bax/Bcl-2 protein expression. [Conclusion] Diabetic myocardial damage was associated with NO, Bax and Bcl-2 protein. Shenqi Compound Prescription can increase NO level, increase the expression of Bcl-2 protein and inhibit the Bax protein expression of myocardium.
出处
《天津中医药》
CAS
2009年第6期489-492,共4页
Tianjin Journal of Traditional Chinese Medicine
关键词
参芪复方
GK大鼠
糖尿病
心肌损伤
心肌细胞凋亡
Shenqi Compound Prescription GK rats diabetic mellitus myocardial damage myocardial apoptosis experimental research treatment of traditional Chinese medicine