CYR61和CTGF在肾盂输尿管连接部梗阻的狭窄段输尿管中的表达

Expression of CYR61 and CTGF in the stenotic tissue of ureteropelvic junction obstruction

目的研究先天性肾盂输尿管连接部梗阻（ureteropelvic junction obstruction，UPJ）的狭窄段输尿管中CYR61和CTGF的mRNA转录和蛋白表达情况及意义。方法采用RT-PCR和western blot技术，分别检测CYR61、CTGF在12例正常输尿管和38例不同梗阻程度的UPJ狭窄段输尿管中的mRNA转录和蛋白表达情况。结果CYR61和CTGF在UPJ中的mRNA转录和蛋白表达均低于正常组织（P〈0．05），其中CYR61的表达水平在重度梗阻与轻、中度梗阻之间差别均有统计学意义（P〈0．05），但轻度梗阻与中度梗阻之间差别无统计学意义（P〉0．05）；而CTGF的表达水平则与梗阻程度无关（P〉0．05）。结论CYR61和CTGF在UPJ中均低表达，CYR61与梗阻程度密切相关，梗阻越重，表达越低；而CTGF与梗阻程度无关。CYR61和CTGF可作为一种新的UPJ诊断和预后的标志物。

Objective To evaluate the transcriptional and translational expression of CYR61 and CTGF in the stenotic tissue of congenital pelvi-ureteric junction obstruction （UPJ）. Methods Tran scriptional and translational expression of CYR61 and CTGF were determined by RTPCR and western blot in stenotic tissue of 38 UPJ patients. Ureters from 12 patients without UPJ were taken as controls. Results The mRNA and protein expression of CYR61 and CTGF in stenosis tissue of UPJ was lower than that of control ureters （P〈0. 05）. The expression of CYR61 was lower in severe UPJs than that in mild to moderate UPJs （P〈0. 05）. But no difference was noted between the mild and moderate UPJs （P〉0.05）. There was no correlation between the expression of CTGF and the severi ty of UPJ （P〉0.05）. Conclusions The expressions of CYR61 and CTGF in UPJ are lower than those of normal ureters. Decreased expression of CYR61 may be associated with increased severity of UPJ.