抗P68抗体检测在类风湿关节炎患者血清中的意义

The expression and clinical significance of anti-P68 antibody in patients with rheumatoid arthritis

目的建立抗P68抗体的检测方法,研究其在类风湿关节炎(RA)中的表达及临床意义。方法以Hela细胞为抗原基质,采用间接免疫荧光法检测71例RA患者,42例其它风湿病患者和30例正常对照组血清中抗P68抗体表达。结果抗P68抗体诊断RA的敏感性和特异性分别为70.42%、76.20%,其在RA组的阳性率明显高于阴性组,差异有统计学意义(P<0.05),抗P68抗体阳性RA组患者血小板计数高、病程长、晨僵持续时间长、握拳差、骨质侵蚀严重。结论抗P68抗体在RA组中有较高的特异性和敏感性,可作为诊断RA的一项特异性血清学指标,该抗体可在RA早期出现,能预测骨质侵蚀、反映疾病的活动性和判断预后。

Objective To evaluate the method of anti-p68 antibody in rheumatoid arthritis and to analyze the clinical significance of anti-p68 antibody of RA.Methods Anti-p68 antibody was tested in the serum of 71 RA patients,42 other rheumatic diseases and normal controls by indirect immunofluorescence （IIF）.The antigen was 68Kda antigen which expressed in Hela cells.Results The positive rate of anti-p68 antibody was higher than that in other rheumatic diseases and none in normal controls.Its sensitivity was 70.42% and specificity was 86.11% in RA.Diseases duration,morning stiffness and making fists in RA patients with anti-p68 antibody positive or negative were different （P﹤0.05）.Conclusion Anti-p68 antibody has statistical sensitivity and specificity,it can be a new and specific serum maker for diagnosis of RA,and it can forecast bone erosion and correlate with disease activity.