非小细胞肺癌中VEGF和MMP-2的表达及其与肿瘤侵袭转移关系的研究

The study of the relationship between the expression of VEGF and MMP-2 and the tumor incursion metastasis of non-small cell lung cancer

目的探讨非小细胞肺癌中血管内皮生长因子(VEGF)和基质金属蛋白酶-2(MMP-2)的表达水平以及与非小细胞肺癌侵袭转移恶性表型形成的关系。方法对60例随访6-64个月的原发性非小细胞肺癌术后肺癌组织石蜡切片,同时取癌旁正常肺组织,采用免疫组织化学SP法进行VEGF和MMP-2表达水平的检测。结果VEGF、MMP-2阳性表达率分别为68.3%和65.0%,与癌旁正常肺组织比较差异有统计学意义(P<0.01)。与肿瘤大小、临床分期呈正相关,差异有统计学意义(P<0.05)。有淋巴结转移、复发转移患者两者的表达强度显著高于淋巴结无转移、无复发转移患者,差异有统计学意义(P<0.05)。两者在肺癌组织中的表达与病人年龄、性别、有无吸烟习惯、组织病理类型均无关(P>0.05)。结论VEGF和MMP-2在非小细胞肺癌组织中的表达均与肿瘤大小、临床分期、有无淋巴结转移、有无复发转移有关。两者在肺癌组织中的表达具有相似性并显著正相关,可作为判断NSCLC中肿瘤细胞侵袭转移力的预见性指标,对判断其恶性程度其有参考价值。

Objective Abstract Objective To probe into the relationship of the expression level of VEGF and MMP-2 and the shaping of tumor incursion metastasis and malignant phenotype for non-small cell.Methods 60 patients were diagnosed as primary non-small cell lung cancer （NSCLC） with clear-cut pathologic diagnosis and competed pathological data and visited for 6-64 months and whose postoperative lung cancer tissue paraffin section and near cancer part normal lung tissue paraffin section were determined for the expression level of vascular endothelial growth factor （VEGF） and matrix metalloproteinase-2 （MMP-2） with immunohistochemistry SP.Results The positive rates of VEGF and MMP-2 were 68.3% and 65.0%,respectively.Comparing with the cancer side normal tissue,there was significant difference （P0.01）.The positive expression rate of VEGF and MMP-2 had positive correlation with clinical pathological stage and the size of the tumor （P0.01）,and was both significantly higher in patients with lymph node metastasis and recurred cancer than in those without （P0.01）.VEGF and MMP-2 were found of no relationship with age,gender,histological and habit of smoking （P0.05）.An obviously positive linear correlation was found between expression of VEGF and MMP-2 （P0.01）.Conclusion The expression of VEGF and MMP-2 is related to the tumor size,clinical pathological stage,lymph node metastasis and reoccurred cancer.VEGF and MMP-2 is closely related with characterization of the invasion and metastasis of NSCLC.VEGF and MMP-2 is also valuable for judging the malignant degree for tumor.