HPLC法测定秦艽中龙胆苦苷含量及药动学的研究

HPLC Determination of Gentiana gentiopicrin content and pharmacokinetic study

目的研究秦艽中龙胆苦苷在家兔体内的药代动力学规律。方法以秦艽水煎醇沉液家兔耳静脉注射后定时取血,以SHIM-PACKVP-ODS色谱柱(150mm×4.6mm,5μm),测定血浆药物浓度,采用DAS软件计算药动学参数。结果龙胆苦苷血药浓度在0.683-136.6μg/ml内线性关系良好(r=0.9995);日内、日间精密度均小于10%,回收率为89.8%-101.4.3%。药-时曲线符合三房室模型,主要药动学参数:Tmax=0.083h,Cmax=45.1mg/L,V=1.312L/kg,CL=0.761L/(h.kg),t1/2=1.195h,AUC(0-∞)=30.78mg/(L.h)。结论该方法具有准确、简便、快速的特点,可用于秦艽中龙胆苦苷的检测。

Objective To establish a HPLC method for the determination of gentiopicrin in rabbits plasma and to investigate the pharmacokinetics of gentiopicrin in gentiana.Methods The analysis was performed on a SHIM-PACK VP-ODS column （150mm×4.6mm,5μm ）.The plasma samples were collected after intravenous administration of the gentiana macrop hyllain rabbits.The concentrations of gentiopicrin in p lasma were measured by HPLC method and the pharmacokinetic parameters were calculated by DAS.ResultsThe calibration curve was linear over the range of 0.683μg/ml—136.6μg/ml （r=0.9995） and the quantitative limit was 0.0342μg/ml.The intra and inter assay coefficient of variation were less than 10 % at all levels.The recoveries were 89.8%—101.4%.The plasma concentration time course followed 3 compartment open model and its main pharmacokinetic parameters were as follows：Tmax=0.083h,Cmax=45.1mg/L,V=1.312L/kg,CL=0.761 L/（h·kg）,t1/2 = 1.195h,AUC（0-∞）=30.78mg/（L·h）.Conclusion This method is sensitive and stable.It is suitable for the pharmacokinetic study of gentiopicrin in gentiana.