摘要
利用玉米芯生产粗木聚糖,再用球毛壳菌ACCC30566木聚糖酶液酶解粗木聚糖,获得低聚木糖,最后对低聚木糖进行提取纯化,并用薄层层析法初步测定了低聚木糖成分。结果表明:球毛壳菌AC-CC30566木聚糖酶酶解产物主要成分为木二糖、木三糖、木四糖等低聚木糖,葡萄糖含量较少。该菌株所产木聚糖酶成分较纯,纤维素酶含量较低。可以用来生产低聚木糖。
Xylooligosaecharide was hydrolyzed from crude xylan produced from corncobs by xylanase ot ACCC30566 strain of Chaetorniurn globosurn and the xylooligosaccharide composition was determined by thin layer chromatography. The results showed that the main composition of enzymatic products by xylanase of ACCC30566 strain of Chaetorniurn globosum were xylobiose, xylotriose, xylotetraose and a little glucose. The composition of xylanase produced by ACCC30566 stain is purity and its cellulose content is lower. Therefore, the ACCC30566 strain of Chaetorniurn globosurn can be used in production of Xylooligosaccharide.
出处
《贵州农业科学》
CAS
北大核心
2009年第12期126-129,共4页
Guizhou Agricultural Sciences
基金
贵州工业大学科研项目"应用现代生物技术提高酶法生产低聚木糖纯度的研究"(2004-308)
贵州大学研究生创新基金项目"毛壳菌酶法水解玉米芯生产低聚木糖的研究"(校研农2006008)
关键词
玉米芯
毛壳菌
木聚糖
木聚糖酶
低聚木糖
corncob
Chaetomiurn globosurn
xylan
xylanase
xylooligosaccharide
