小鼠皮肤切创愈合过程中FAP和α-SMA的表达

Expression of FAP and α-SMA During the Incised Wound Healing in Mice Skin

目的观察小鼠皮肤切创愈合过程中成纤维细胞激活蛋白(fibroblast activation protein,FAP)和α-平滑肌肌动蛋白(α-smooth muscle actin,α-SMA)表达的变化规律。方法应用免疫组织化学和West-ern印迹法检测小鼠皮肤切创后各个时间段FAP和α-SMA表达情况。结果免疫组化结果显示FAP与α-SMA在正常对照组及伤后1h组小鼠皮肤弱表达,伤后6h阳性细胞率开始升高,伤后5dFAP阳性细胞率达高峰,伤后3dα-SMA阳性细胞率达高峰,伤后14dFAP与α-SMA恢复至与对照组相同。Western印迹法检测显示FAP和α-SMA从1d起各个时间段均有表达,其中5d为FAP的表达高峰,3d为α-SMA的表达高峰。结论FAP可作为法医学皮肤切创形成时间的推断指标,α-SMA可作为损伤修复中后期的推断指标,FAP与α-SMA联合使用有望成为推断皮肤损伤时间的有效指标。

Objective To investigate the time-dependent expression of fibroblast activation protein（FAP） and α-smooth muscle actin（α-SMA） during the incised wound healing of the skin in mice. Methods The expression of FAP and α-SMA in incised wound of mice skin was detected by immunohistochemistry and Western blot. Results By immunohistochemistry, the expression of FAP and α-SMA in the normal skin and the skin 1 h after injury maintained at a very low level, but the positive cells expressing FAP and α-SMA started to elevate 6 h after injury and reached its peak on 5 d for FAP and on 3 d for α-SMA, then gradually decreased to the normal level on 14 d. The expression of FAP and α-SMA was observed throughout the wound healing stages 1 d after injuries by Western blot as well with a peak expression occurring on 5 d for FAP and on 3 d for α-SMA after injury. Conclusion FAP may be a potentially useful marker for wound age determination and α-SMA may be used as an effective indicator for the mid-and late stage incised wound of mice skin. The combination use of FAP and α-SMA may be potentially effective indicators for wound age determination.