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Patatin启动子驱动的人胰岛素原基因的植物表达载体的构建

Construction of Human Proinsulin Gene Plant Expression Vector Controlled by Patatin Promoter
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摘要 使用马铃薯的偏爱密码子设计合成人胰岛素原基因,以植物表达载体pCAMBIA1301为基础,构建由patatin启动子调控的人胰岛素原基因植物表达载体p1301Ph,并将导入根癌农杆菌LBA4404中,经PCR鉴定证明,此基因已整合到农杆菌Ti上。表达载体的建立为日后转基因工作奠定了基础。 Human proinsulin gene was designed and synthesized with the potato partial codon. Plant expression vector p1301Ph was derived from pCAMBIA 1301, with human proinsulin gene being controlled by patatin promoter. Then the recombination plasmid was introduced into Agrobacterium LBA4404. It was proven that the vector was integrated into LBA4404. This study provides a foundation for the transgenic of plants.
作者 马建忠 马雪青 王永刚 宋国伟 周贤靖 王倩
机构地区 兰州理工大学生命科学与工程学院
出处 《中国马铃薯》 2009年第6期332-337,共6页 Chinese Potato Journal
基金 甘肃省自然基金马铃薯块茎生产天然人胰岛素的研究资助(3ZS062-B25-023)
关键词 patatin启动子 人胰岛素原基因 植物表达载体 patatin promoter human proinsulin plant expression vector
分类号 S532 [农业科学—作物学] Q578 [生物学—生物化学]
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参考文献8

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二级参考文献34

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共引文献6

中国马铃薯
2009年 第6期

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