西北部分地区无偿献血人群HCV感染的分子流行病学的研究

Molecular epidemiological studies of the HCV infection among volunteer blood donors in partial northwest regions of China

目的探讨西北部分地区无偿献血人群中丙型肝炎病毒(HCV)基因型(亚型)的分布。方法收集无偿献血人群中HCV RNA阳性标本,通过RT-PCR扩增NS5B基因,测定其核苷酸序列后,与Genebank中已知序列进行分子进化分析,确定HCV基因型(亚型)。结果对西北部分地区(新疆、陕西、青海)137例HCV RNA阳性血清进行NS5B基因扩增,扩增阳性128例,其中1b占43.00%(55/128),2a占35.16%(45/128),3a占9.38%(12/128),3b占10.16%(13/128),6a占2.34%(3/128)。1b亚型之间同源性最高为99%,最低为93%,2a亚型之间同源性最高为98%,最低为91%,3a亚型之间同源性最高为98%,最低为91%,3b亚型之间同源性最高为98%,最低为93%。结论西北部分地区无偿献血人群中HCV基因分型以1b和2a为主,3a,3b,6a亚型占的比例较低,和中国内陆地区的基因型分布相似。

Objective To determine the distribution of HCV genotypes among volunteer blood donors in partial northwest regions of China. Methods A total of 137 RNA positive samples were collected from volunteer blood donors in Xinjiang, Shanxi and Qinghai provinces. NSSB fragments of HCV were amplified,followed by DNA sequencing and phylogenetic analysis. Results HCV genotypes were determined for 128 samples. And among them, subtypes lb, 2a, 3a, 3b, 6a were detected with frequencies of 43.00%, 35. 16%, 9.38%, 10. 16% and 2. 34%, respectively. The highest and the lowest homologies among lb, 2a, 3a and 3b were 99% vs 93%, 98% vs 91% , 98% vs 91%, and 98% vs 93%, respectively. Conclusion HCV lb and 2a were the most predominant subtypes among volunteer blood donors in partial northwest regions of China.