摘要
目的了解广州地区首次参加无偿献血人群HCV的感染状况。方法抗-HCV检测采用酶联免疫吸附实验(使用2种不同厂家的试剂盒),HCV RNA检测采用荧光定量PCR法,ALT检测采用速率法。结果2004—2007年在广州血液中心首次参加无偿献血的559890名献血者中,1617名经双试剂检测抗-HCV阳性,阳性率为0.289%。随机选取435名双试剂检测抗-HCV阳性血液标本进行RNA检测,266名阳性,占61.15%,男女(P<0.01)HCV RNA阳性率有统计学意义(P<0.01)。266名抗-HCV检测阳性献血者中,ALT升高(>40U)的为18名(其中男性17名),不合率为6.77%,男女ALT的不合格比有统计学意义(P<0.01)。结论随着无偿献血的开展,献血人群中HCV的阳性率不断下降;不同性别的个体清除HCV的能力不同,女性高于男性。
Objective To investigate the prevalence of HCV infection among first-time volunteer blood donors recruited during 2004--2007 in Guangzhou. Methods Anti-HCV was detected using two different third-generation EIA kits. HCV RNA was detected using fluorescent quantitat;on polymerase chain reaction (Q-PCR). ALT was detected by the speed rate method. Results A total of I 617(0. 289% ) were positive for anti-HCV with two kits. Among them, 435 were selected randomly for HCV nucleic acid amplification by Q-PCR, and 266(61.15% ) were HCV RNA ( + ). The HCV RNA( + ) rate was significantly higher in males than in females (66.89% vs 49. 30% , P 〈0. 01 ). Among the 266 HCV RNA ( + ) donors, 18 showed abnormal ALT level ( 〉40 U). The abnormal ALT rate was higher in males than females (8.67% vs 1.43% ) ,without any statistical difference (P 〉0.05). Conclusion The anti-HCV( + ) rate among blood donors in China reflects a steady decreasing tendency, owing to the current recruitment of exclusively volunteer donors. Spontaneous clearance of HCV was more likely to occur in females than males.
出处
《中国输血杂志》
CAS
CSCD
北大核心
2009年第11期883-885,共3页
Chinese Journal of Blood Transfusion
基金
国家自然科学基金(编号:30872162)
广东省自然科学基金(编号:6021796)
广州市医药卫生重点项目(2006-ZDi-12
2008-ZDi-10)资助