剑叶龙血树愈伤组织的诱导及其增殖培养研究

The Callus Induction and Proliferation of Dracaena cochinchinensis

以西双版纳可提取药用活性成分"血竭"的剑叶龙血树为材料,进行愈伤组织的诱导及增殖培养,结果显示:外植体的消毒侧芽以75%乙醇浸泡2 min+0.1%升汞浸泡8 min,花蕾以75%乙醇浸泡1.5 min+0.1%升汞浸泡8 min,叶片以75%乙醇浸泡3 min+0.1%升汞浸泡12 min效果较好;愈伤组织诱导以花蕾为外植体效果较好,且诱导与增殖的培养基为B5+2.0 mg/L NAA+0.5 mg/L6-BA.

The induction and proliferation of callus is done by using the plant from Xishuangbanna Dracaena cochinchinensis from which extracted medicinal active ingredients ＂Resina Draconis＂ as the material. The results showed that 2 min soaking in 75% ethanol plus 8min in 0.1% HgCl2 were the best sample treatment for buds, the same as 1.5 min in 75% ethanol plus 8 min in 0.1% HgCl2 for budet and 3min in 75% ethanol plus 12min in 0. 1% HgCl2 for leaves. The budet is the best material for callus induction of D. cochinchinensis and the optimal culture media to induction and proliferation is B5 ＋ 2.0 mg/L NAA ＋ O. 5 mg/L 6 -BA.