摘要
目的观察中风皂贝化痰胶囊对脑缺血再灌注损伤后的神经保护作用及对caspase-3表达的影响。方法SD大鼠50只随机分成假手术组、缺血再灌注组、中风皂贝化痰胶囊大剂量组、中剂量组和小剂量组,每组10只。采用ZeaLonga法制作脑缺血再灌注大鼠模型,观察中风皂贝化痰胶囊对大鼠脑缺血再灌注损伤后的神经功能症状,以及对caspase-3表达的影响。结果与假手术组相比,缺血再灌注组大鼠出现严重的神经功能缺失症状,皮质和海马区caspase-3表达增强;与脑缺血再灌注组相比,中风皂贝化痰胶囊治疗组能显著改善大鼠神经功能缺失症状,降低caspase-3的表达,其中以中风皂贝化痰胶囊大剂量组的神经保护作用最为显著(P<0.01)。结论中风皂贝化痰胶囊对大鼠脑缺血再灌注损伤具有保护作用,其作用机制可能与抑制caspase-3表达相关。
Objective To observe the effect of Zhong Feng Zao Bei Hua Tan Jiao Nang （ZFZBHTJN） on nerve protection and the expression of caspase - 3 in the rats with cerebral ischemia - repeffusion injury. Methods 50 SD rats were randomly divided into sham operation group, ischemia - reperfusion （I -R） group, ZFZBHTJN high -dose group and ZFZBHTJN middle -dose group and ZFZBHTJN low - dose group, 10 rats in each group. The rat model of cerebral ischemia - reperfusion was prepared by Zea Longa method. The impacts of ZFZBHTJN on neurologic function and the expression of caspase - 3 were ob- served. Results Compared with sham operation group, I - R group presented severe neurological deficits and the increase of caspase - 3 expression in cortex and hippocampus. Compared with I - R group, neurological deficits of rats were relieved significantly and caspase -3 expression decreased in ZFZBHTJN groups, of which ,the high -dose group presented the most significant effect on nerve protection（ P 〈 0.01 ）. Conclusion Zhong Feng Zao Bei Hua Tan Jiao Nang acts on the protection of cerebral ischemia - reperfusion injury in rats,which is probably correlated with its inhibition on caspase- 3 expression.
出处
《世界中西医结合杂志》
2009年第12期862-864,共3页
World Journal of Integrated Traditional and Western Medicine
基金
河南省教育厅教育攻关项目(No.2007360031)
关键词
中风皂贝化痰胶囊
脑缺血再灌注损伤
凋亡
CASPASE-3
大鼠模型
Zhong Feng Zao Bei Hua Tan Jiao Nang （ZFZBHTJN）
Cerebral ischemia - reperfusion injury
Apoptosis
Caspase - 3
Rat model
