RP-HPLC法测定大鼠血浆中姜黄素含量

Determination of curcumin in rat plasma by RP-HPLC

目的 采用RP-HPLC法测定大鼠血浆中姜黄素的含量。方法血浆样品经处理后，按以下色谱条件测定姜黄素的含量：DiamonsilC18柱（4．6mm×250mm，5μm），柱温：室温；流动相：乙腈-2％醋酸溶液（58：42）；流速：1．0ml／min；检测波长：426nm．结果在本色谱条件下血浆中的杂质峰较少，姜黄素和内标物及其他内源性干扰成分可成功分离。采用乙酸乙酯液液萃取法处理血浆样品，萃取回收率最高。在0．1～0．5mg／L浓度范围内，线性关系良好，回归方程y=0．0026x-0．0756，r=0．9984；日内、日间精密度RSD符合生物样品的分析要求；加样回收率和萃取回收率均在90％以上。结论所建立的测定大鼠血浆中姜黄素含量的方法简单可行、准确可靠，适用于姜黄素的血药浓度监测。

Objective To develop a RP-HPLC method for the determination of eurcumin in rats plasma. Method After the plasma was treated, the samples were separated well on a DiamonsilC18 column（4.6 mm × 250 mm, 5μm） under room temperature;the mobile phase was aeetonitrile： 2 % acetic acid solution（58 ： 42） at a flow rate of 1.0 ml/ min; the detective wavelength was 426 nm. Results Curcumin could be successfully separated from internal standard substance and other endogenous interference components under the chromatographic conditions. Liquid-liquid extraction method adopted to treat the plasma samples had the highest recovery rate. Between 0. 1 and 0. 5 mg/L, the method had a good linear relationship, while the regression equation was y=0. 0026x-0. 0756, r=0. 9984;the within-day and between-days precision RSD were less than 10%;the average method recovery and the average extraction recovery were more than 90%. Conclusion The RP-HPLC method for the determination of curcumin in rat plasma is accurate and reliable,which could be applied to determine the curcumin concentration in the blood.