中国国内首次分离出EV73肠道病毒

Molecular Identification of Human Enterovirus 73 in Yunnan Province,The People's Republic of China

对云南省与缅甸接壤边境地区健康儿童粪便标本中分离到的2株病毒进行VP1基因序列测定,以了解其分子生物学特点。用肠道病毒通用引物292/222进行RT-PCR,并对扩增产物进行测序。将基因序列提交GenBank进行BLAST搜索,同时运用Mega软件进行同源性分析,并构建基因树。核苷酸和氨基酸同源性比较结果证明,2株病毒为EV73型,为国内首次报道。

Molecular typing was conducted according to the reported methods for 2 enteroviruses that were isolated from healthy children in the border areas of Yunnan Province with Myanmar. RT-PCR and sequencing were performed with 292/ 222 primers according to the Oberste＇s methods. The resulting sequences were blasted against the Genbank database and compared with all available enterovirus database. Analysis of homology at nucleotide and amino acid level identically suggested that the two enteroviruses are human enterovirus 73.