白藜芦醇对单核细胞表达CC类趋化因子受体2基因和内皮细胞分泌单核细胞趋化蛋白1的影响

Effects of Resveratrol on Expression of CC Chemokine Receptor 2 Gene in Monocytes and Secretion of Monocyte Chemoattractant Protein-1 in Endothelial Cells

目的探讨白藜芦醇对活化的人脐静脉内皮细胞分泌单核细胞趋化蛋白1及对人单核细胞株THP-1细胞表面CC类趋化因子受体2基因表达的影响。方法半定量逆转录聚合酶链反应法检测THP-1细胞CC类趋化因子受体2mRNA的表达;以肿瘤坏死因子α激活人脐静脉内皮细胞,酶联免疫吸附法测定活化的人脐静脉内皮细胞单核细胞趋化蛋白1的分泌。结果10μmol/L和50μmol/L白藜芦醇可以抑制THP-1细胞CC类趋化因子受体2mRNA的表达(0.19±0.02和0.06±0.02比0.73±0.15,P<0.01),且随着白藜芦醇剂量的升高(1、10、50μmol/L),基因表达抑制也逐渐加强(P<0.01);10μmol/L和50μmol/L白藜芦醇可减少活化的人脐静脉内皮细胞单核细胞趋化蛋白1的分泌(9663.33±927.38ng/L和2822.17±472.47ng/L比16595.67±1667.39ng/L,P<0.01),随着白藜芦醇剂量升高,单核细胞趋化蛋白1分泌逐渐减少(P<0.01)。结论白藜芦醇能剂量依赖性地抑制单核细胞CC类趋化因子受体2基因的表达,并减少活化的内皮细胞单核细胞趋化蛋白1的分泌,从而有效抑制单核细胞的趋化,发挥抗动脉粥样硬化的作用。

Aim To investigate the effect of resveratrol on the secretion of monocyte chemoattractant protein-1（MCP-1） in activated human umbilical vein endothelial cells（HUVEC）,and the expression of CC chemokine receptor 2（CCR2） gene in human monocyte cell line THP-1 cells.Methods The expression level of CCR2 gene was measured by semiquantitative reverse expression polymerase-chain reaction in THP-1 cells.HUVEC were activated with tumor necrosis factor-α after preincubation of resveratrol.Then the secretion of MCP-1 in the cultivate supernatant of HUVEC was investigated by enzyme linked immunosorbent assay.Results The expression of CCR2 gene was inhibited by 10 μmol/L and 50 μmol/L resveratrol in monocyte （0.19±0.02 and 0.06±0.02 vs 0.73±0.15,P〈0.01）.The inhibition was increased with the dosage of resveratrol（1 μmol/L,10 μmol/L and 50 μmol/L,P〈0.01）.The secretion of MCP-1 protein in activated HUVEC was suppressed by 10 μmol/L and 50 μmol/L resveratrol （9 663.33±927.38 ng/L and 2 822.17±472 .47 ng/L vs 16 595.67±1 667.39 ng/L,P〈0.01）.The suppression was enhanced with the increased dosage of resveratrol（P〈0.01）.Conclusion Resveratrol can inhibit the expression of CCR2 gene in THP-1 cell and suppress the secretion of MCP-1 in activated HUVEC.Both effects are in a dose-dependent manner,which may actively suppress chemotaxis of mononuclear cells.It may contribute to resveratrol＇s anti-atherosclerosis effects.