Science Letters:Determination of benzalkonium chloride in viscous ophthalmic drops of azithromycin by high-performance liquid chromatography

A high-performance liquid chromatography （HPLC） system was used in the reversed phase mode for the determination ofbenzalkonium chloride （BKC） in azithromycin viscous ophthalmic drops. A Venusil-XBP（L）-C18 （150 mm×4.6 mm, 5 gm） column was used at 50℃. The mobile phase consisted of a mixture of methanol-potassium phosphate （16：5, v/v）. Two sample preparation methods were compared. The results suggested that, compared with an extraction procedure, a deproteinization procedure was much quicker and more convenient. Using the deproteinization procedure for sample preparation, calibration curves were linear in the range 5.0-50μg/ml. The within-day and inter-day coefficients of variation were less than 10%. The average recoveries were determined as 96.70%, 98.52%, and 97.96% at concentrations of 10.0, 30.0, and 50.0 μg/ml, respectively. Variability in precision did not exceed 5%. In conclusion, this HPLC method using a simple sample treatment procedure appears suitable for monitoring BKC content in azithromycin viscous ophthalmic drops.

一个高效的液体层析(HPLC ) 系统在 azithromycin 为 benzalkonium 氯化物(BKC ) 的决心在颠倒的阶段模式被使用粘滞眼的落下。Venusil-XBP (L)-C18(150 公里× 4 .6 公里， 5 μ m ) 列在 50 ° C 被使用。活动阶段由甲醇钾磷酸盐的混合物组成了(16:5， v/v ) 。二个样品准备方法被比较。结果建议与一个抽取过程相比，一个 deproteinization 过程快得多、更方便。用为样品准备的 deproteinization 过程，刻度曲线在范围是线性的 5.0 ∼50 μ g /ml。在内天和变化的内部天的系数是不到 10% 。平均恢复分别地在 10.0， 30.0，和 50.0 μ g /ml 的集中作为 96.70% ， 98.52% ，和 97.96% 被决定。在精确的可变性没超过 5% 。在结论，用一个简单样品处理过程的这个 HPLC 方法对在 azithromycin 监视 BKC 内容显得合适粘滞眼的落下。