基于棉花逆转座子的SSAP标记的开发

Development of a Retrotransposon-based SSAP Marker in Cotton(Gossypium spp.)

从棉花BAC序列中鉴定了1个Ty1-copia类逆转座子,根据RNAseH-LTR连接区序列信息设计了特异引物,通过优化试验条件建立了一套经济、高效的SSAP(Sequence-specific amplification polymorphism)标记体系。分析棉属不同种的SSAP发现,SSAP在棉属不同种中均有丰富的扩增位点,而且棉花中SSAP多态性位点远高于AFLP(Fragment length polymorphic markers)。这一标记系统可增加棉花染色体端粒区域的标记密度,促进棉花高密度遗传图谱的构建。

A Tyl-copia retrotansposon was identified from a cotton BAC sequence. Specific primers were designed based on RNase-LTR junction sequence. SSAP （Sequence-specific amplification polymorphism） analysis system was developed and applied in seven Gossypium species. All primer combinations amplified visible products in seven tested species. The results revealed that SSAP had higher level of polymorphism than amplified fragment length polymorphic markers （AFLP） in cotton. The study suggests that the SSAP marker system has potential for facilitating in constructing saturated genetic mapping in cotton.