摘要
目的:通过超临界萃取蚕蛹提取物制作天鹿胶囊,并观察其对小鼠的抗运动疲劳能力的影响。方法:选用昆明种健康小鼠,雌雄各半,体重18~22g,按阴性对照组,阳性对照组,天鹿胶囊低剂量、中剂量、高剂量给药组随机分为5组,分别观察小鼠游泳试验结果并测定抗疲劳活性物质含量。结果:灌胃给药20d后,天鹿胶囊高中低三个剂量组均明显促进小鼠的体重增长,与溶剂对照组比较有显著性差异(P〈0.05)。天鹿胶囊1.2,1.8g/kg组均能显著延长小鼠负荷游泳时间,与溶剂对照组比较有统计学差异(P〈0.01)。天鹿胶囊1.2g/kg,1.8g/kg两个剂量组均能明显降低血清中肌酸激酶活性和尿素氮,乳酸含量,1.8g/kg组还可显著提高雄性和雌性小鼠血清睾酮的含量,与溶剂对照组相比有统计学差异(P〈0.05)。天鹿胶囊1.8g/kg剂量组对血清中胆固醇和甘油三酯的含量均有明显降低作用,与溶剂对照组相比有统计学差异(P〈0.05)。天鹿胶囊三个剂量组能明显提高小鼠力竭游泳后肝糖元含量,与溶剂对照组相比有显著性差异。结论:天鹿胶囊具有明显的抗疲劳作用,通过脂肪代谢和提高糖原储备而增加运动时供能。
Objective: Observe the effect of the anti - sports - fatigue ability of mouse with Tianlu capsule which produced by supercritical fluid silkworm chrysalis extractive. Methods:Healthy mice (BW:18 -22g)were selected and divided into 5 groups: negative, positive, low dose Tianlu capsule, median dose, high dose, it was to observe the swimming test results and test the contains of anti -fatigue active substance..Results :The BW were rising in low, median and high dose group, and was significantly different with control group after intragastric feeding 20 days (P 〈 0.05 ). The median and high dose group can obviously prolong the load swimming time( P 〈 0.01 )and can significatly decrease the contain of serum CK, usea nitrogen and LA (P 〈 0. 05 ). The TES was improved ( P 〈 0.05 ), the cholesterol and TG were decreased in high dose group ( P 〈 0.05 ). The muscle glycogen was increased after exhausted swimming in low, median and high dose group (P 〈 0. 05 ). Conclusion: Tianlu capsule has anti - fatigue effect and can improve the energy supply during exercise by fat metabolism and improving glycogen storage.
出处
《山东体育学院学报》
北大核心
2009年第11期43-45,共3页
Journal of Shandong Sport University
基金
山东省科技厅科技发展计划项目(2008GG30001026)
关键词
蚕蛹提取物
运动性疲劳
鹿茸
小鼠
silkworm extractive
sports fatigue
deer horn
mouse