唾液乳杆菌抑制镰孢霉的研究

Antagonism of Lactobacillus salivarius ssp. salivarius ZDY162a against Fusarium

目的研究唾液乳杆菌抑制产毒镰孢霉的生物学性能,初步探索抑菌机制。方法以禾谷镰孢霉和尖孢镰孢霉2种典型霉菌为指示菌,唾液乳杆菌为测试对象,对霉菌孢子萌芽、孢子生长和菌丝体生长3个生理阶段进行抑制效应观察。结果10%的唾液乳杆菌耗尽上清就能抑制83%的禾谷镰孢霉孢子和50%尖孢镰孢霉孢子萌芽;耗尽上清24 h内能显著抑制镰孢霉孢子的生长;96 h内孢霉菌丝体的生长。结论唾液乳杆菌产生的有机酸对禾谷镰孢霉和尖孢镰孢霉生长起主要抑制作用。

Objective To study the antagonism and mechanism of Lactobacillus Salivarius ssp.salivarius ZDY162a against mytoxin-producing Fusarium.Method Fusarium graminearum and Fusarium oxysporum were adopted as the indicator strains to test the inhibition effect of metabolites of L.salivarius ssp.salivarius ZDY162a on spore germination,spore growth and mycelium growth.Result 10% of the spent culture supernatant（SCS） of L.salivarius ssp.salivarius ZDY162a could inhibit the spores germination of Fusarium graminearum by 83% and Fusarium oxysporum by 50%,respectively.The SCS inhibited remarkably the growth of spores in 24 h and that of mycelia of Fusarium in 96 h.Conclusion Some organic acids produced by L.salivarius ssp.salivarius ZDY162a were probably playing an primarily role in inhibiting the growth of Fusarium graminearum and Fusarium oxysporum.