乙型肝炎病毒感染HepG2细胞模型的构建

Establishment of hepatitis B virus-transfected HepG2 cell model

目的:构建乙型肝炎病毒(HBV)感染的细胞模型。方法:将携带1.2拷贝HBV基因的重组腺病毒感染HEK293细胞进行包装、扩增并分离纯化。将扩增得到的HBV感染HepG2细胞,用ELISA法检测感染后第4天培养上清中的HBsAg,Real-time RT-PCR检测HBV mRNA。结果:ELISA检测结果显示,HepG2细胞感染HBV后上清液中HBsAg呈阳性。Real-time RT-PCR可以检测到HBV mRNA。结论:HBV能在HepG2细胞中表达复制和表达,HBV感染的HepG2细胞模型构建成功。

Objective To establish a cell model of hepatitis B virus （HBV） infection. Methods HEK293 cells were infected with adenovirus containing 1.2 copies of HBV DNA. After replication, the HBV were then isolated, purified and finally used to infect HepG2 cells. The expressions of HBsAg in the supernatant of the culture medium were detected by enzyme-linked immunosorbent assay （ELISA） from days 1 to 7 of HepG2 cell being infected with HBV. HBV mRNA was detected by real-time PCR. Results ELISA revealed HBsAg was positive in the supernatant. HBV mRNA was detectable by real-time RT-PCR. Conclusion HBV gene can replicate and express in HepG2 ceils. HepG2 cell model of HBV infection is successfully established.