摘要
从黄色短杆菌（Ｂｒｅｖｉｂａｃｔｅｒｉｕｍｆｌａｖｕｍ）ＡＴＣＣ１４０６７出发，选育获得一株Ｌ－异亮氨酸高产菌ＺＱ－４．试验结果证明，生物素是ＺＱ－４菌株唯一必需生长因子，生物素或玉米浆用量分别为５０μｇ／Ｌ和２０ｍＬ／Ｌ时可得到较高的产酸率。为了获得较高的Ｌ－异亮氨酸积累量，培养基中葡萄糖质量浓度为１４０ｇ／Ｌ，硫酸铵用量为４０ｇ／Ｌ时较为适宜；以适当的摇瓶装液量改善通风状况可明显提高产酸率。在适宜的发酵条件下，ＺＱ－４经摇瓶发酵培养３ｄ，Ｌ－异亮氨酸积累量可达２８～３０ｇ／Ｌ．
Studies on the fermentation of L isoleucine were carried out by using a L isoleucine producing strain ZQ 4 (AHV rAEC rSuc gSG rEth rα AB rIleHx r) with shaking flask. The results showed that biotin was the only essential growing factor, as its father strain Brevibacterium flavum ATCC14067. A certain amount of oxygen supplying was an important factor. Shaken at 31℃for 3 days, the amount reached 28￣30 g/L in the medium containing 140 g/L glucose, 40 g/L (NH 4) 2SO 4 , 1 g/L KH 2PO 4 , 0.5 g/L MgSO 4·7H 2O, 20 mL/L corn steep liquor, and 32 g/L CaCO 3.
