两个新选玉米雄性不育材料的初步鉴定和分析

Preliminary Identification and Analysis of Two New Male Sterile Maize Materials

通过不育稳定性、恢复专效性以及分子特异性分析,对两个新选玉米不育材料"R1"和"R2"进行初步鉴定。结果表明,这两个不育材料均为可遗传的细胞质雄性不育,不育性表现稳定,且能被"恢313"保持,被"自凤1"恢复,特异PCR扩增的带型与C组细胞质雄性不育一致。初步判断这两个不育材料均属于C组细胞质雄性不育。但因它们的恢复系和保持系不尽相同,推测可能属于不同的C亚组。根据恢复株自交F2的分离比例,推断"R1"的育性恢复受2对或3对基因控制,而"R2"的育性恢复受1对显性基因控制。

Preliminary identification of two newly bred male sterile maize materials was performed through analysis of stability of sterility, specific effect of restoration and molecular specification. The results showed that both the two male sterile materials were heritable cytoplasmic male-sterility （CMS）, whose sterility was stable and could be restored by ＂Zifeng 1＂ and maintained by ＂Hui 313＂. Furthermore, the specific PCR amplification bands conformed to that of C group CMS. Thus it was preliminarily concluded that the two sterile materials belonged to the C group CMS. However, because their restorer lines and maintainer lines were not all the same, it was presumed that they might belong to different subgroups of C group. According to the separation ratio of self-cross F2 of the restored plants, it was deduced that the fertility restoration of ＂R1＂ was controlled by two or three genes, while the fertility restoration of ＂R2＂ was controlled by one dominant gene.