法国亮大蜗牛和白玉蜗牛凝集素部分性质的研究

Some Properties of the Lectins from the Helix Lucorum and Achatina Fulica

采用家鸡、家鸽、家兔、虎纹蛙、猪的红细胞测定法国亮大蜗牛凝集素和白玉蜗牛凝集素的凝集活性，进行糖抑制、热稳定性、pH及Ca2＋影响试验．结果表明，法国亮大蜗牛凝集素和白玉蜗牛凝集素都能凝集家鸡、家鸽、家兔、猪4种红细胞，且对兔红细胞的凝集活性最高，对蛙红细胞的凝集活性最低；两者凝集活性在pH7～8最适宜，它们均有一定的热稳定性，部分糖对蜗牛凝集素产生抑制作用，金属离子能影响蜗牛凝集素的凝集效价，蜗牛凝集素的凝集活性依赖于Ca2＋．

Lectins from Helix lucorum and Achatina fulica were tested for agglutinating using erythrocytes of chick, pigeon, rabbit, frog and pig. Besides, glyco-inhibiting, and influence of pH and Ca2＋ to the agglutinative activity of them were tested. The results show that the lectins of Helix lucorum and Achatina fulica will agglutinate four erythrocytes, and their agglutinative activity to the rabbit erythrocyte is the highest,to the frog erythrocyte is the lowest. The agglutination needs pH 7- 8 as fit action conditions and shows certain tolerance to heat treatment. The agglutinative activity can be influenced by EDTA and can be inhibited by partial saccharide. The agglutinative activity of snail lectins depends on Ca2＋.