幽门螺杆菌热休克蛋白基因HspB-C对甘薯遗传转化的研究

Transformation of Heat Shock Protein Gene(HspB-C) of Helicobacter pylori into Sweetpotato Varieties

甘薯(Ipomoea batatas(L.)Lam.)是世界上重要的粮食、饲料和工业原料作物,作为生物反应器具有优势。幽门螺杆菌被世界卫生组织认定为一类致癌因子,其热休克蛋白(HSP)具有较强的免疫原性,存在着发展为疫苗成分的可能性。本研究构建了幽门螺杆菌热休克蛋白基因HspB-C植物表达载体,以根癌农杆菌(Agrobacterium tumefaciens)介导转入4个甘薯品种诱导愈伤和再分化,通过PCR检测证明目的基因HspB-C已成功转化入甘薯品种南瑞苕中。

Sweet potato which was one of the most important crops in the world and had many advantages as a new bioreactor. Helicobacter pylori, as a kind of cancer-causing factor by the World Health Organization, had a strong immunogenicity, and its monoclonal antibody had bactericidal activity, which had the possibility as the vaccine components. In this research, we had constructed the plant expression vector with heat shock protein gene （HspB-C） of Helicobacter pylori. This vector was transformed by Agrobactrium tumefaciens EHA105 into four sweet potato varieties. After callus-induction and re-differentiation, we got the transgenic plants from sweet potato variety of Nancy holl.