摘要
本文采用原位杂交和免疫组织化学技术,观察孤儿受体TR3及其mRNA在小鼠睾丸中的表达及细胞定位。结果表明,在小鼠睾丸中有显著量的孤儿受体TR3mRNA和蛋白表达,其表达量在不同曲细精管有明显的差异;孤儿受体TR3蛋白主要定位于生精细胞,其mRNA在生精细胞特异表达,主要在精原细胞和发育早期的初级精母细胞表达,提示孤儿受体TR3在小鼠曲细精管精子发生的早期阶段中起着调控作用。
By means of in situ hybridization and immunohistochemistry, the expression and cellular localization of orphan receptor TR3 and its mRNA were observed. The results showed that both TR3 and its mRNA were expressed in a significant amount in mouse testis, and the expression level of TR3 mRNA was different in current seminiferous tubules. TR3 mRNA was specifically expressed in germ cells, mainly in spermatogonia and less advanced primay spermaaatocytes, whereas TR3 receptor protein was banlylocalized in germ cells. It is suggested that TR3 may play an important role in regulating the early stage of germ cell development in mice.
出处
《生理学报》
CAS
CSCD
北大核心
1998年第4期439-443,共5页
Acta Physiologica Sinica
基金
中国科学院九五重点项目!KJ952-J1-603
自然科学基金!39670285
国家九五攀登预选项目
关键词
孤儿受体TR3
原位杂交
免疫组织化学
精子发生
orphan receptor TR3
in situ hybridization immunohistochemistry
spermatogenesic