谷胱甘肽S转移酶π高水平表达抑制人类宫颈癌HeLa细胞的生长、转移和浸润

Inhibition of Cervical Cancer Cell Growth,Migration and Invasion by GSTπ Overexpression

目的研究胎盘型谷胱甘肽S转移酶π(GSTπ)在宫颈癌细胞中的高表达对其生物学特性的影响。方法通过脂质体介导转染,在人类宫颈癌HeLa细胞建立GSTπ基因高表达稳定转染株;采用细胞计数和MTT法测定细胞的生长;采用划痕法和Boyden小室法检查细胞的转移、浸润能力;采用RT-PCR和Western blot技术检测宫颈癌细胞中相关mRNA和蛋白的表达水平。结果成功构建了GSTπ高表达和空质粒转染的HeLa细胞株;GSTπ基因高表达明显地抑制HeLa细胞的生长、转移和浸润。结论GSTπ可以明显抑制HeLa细胞的生长、转移和浸润,为GSTπ作为一种新的抑癌基因提供了临床前实验数据和依据。

Objective To study the impact of GSTπ overexpression on human cervix cancer HeLa cells growth,migration and invasion. Methods HeLa cells with GSTπ overexpression were established by stable transfection with a mammalian pcDNA3 expression vector carrying a full-length GSTπ cDNA （pcDNA3/GSTπ）. Cell counting was used to evaluate cell growth; cell migration and invasion was moni-tored by in vitro scratch assay and invasive model-Boyden chamber; GSTπ protein and mRNA expression were determined by Western blot and RT-PCR assay,respectively. Results An increased expression of GSTπ was obtained in stably transfected HeLa cells,with pcDNA3/GSTπ compared to ＂empty＂ pcDNA3 transfected HeLa cells and HeLa parent cells,as determined by Western blot and RT-PCR as-say. A significant inhibition of cell growth,migration and invasion were observed in GSTπ overexpressing HeLa cells,which were related to an up-regulation of cell cycle-associate cyclin D1 and invasion inhibitor PTEN expression. Conclusion These results,for the first time,demonstrate that the increased expression of GSTπ reduces growth,migration and invasion of human cervical cancer HeLa cells,which suggest that the GSTπ gene as a new tumor suppressor gene play an important role in human cervix cancer.