2型糖尿病及大血管病变患者T细胞亚群的变化及其意义

T Lymphocyte Subsets in Type 2 Diabetes and Its Macrovascular Complications

目的观察外周血T淋巴细胞亚群在2型糖尿病(T2DM)患者及大血管病变患者的变化,探讨其在T2DM及大血管病变发病中的作用。方法应用免疫荧光标记技术和流式细胞仪,检测36例初诊T2DM(单纯T2DM组)患者、28例T2DM并发大血管病变患者及20名健康对照者的外周血T细胞亚群变化。结果T2DM并发大血管病变组、单纯T2DM组CD4+T细胞、CD4/CD8比值、CD4+CD28-T细胞明显高于健康对照组(均P<0.05);而CD3、CD8+T细胞、CD4+CD25+T细胞明显低于健康对照组(均P<0.05);T2DM并发大血管病变组CD4+T细胞、CD4/CD8比值、CD4+CD28-T细胞明显高于单纯T2DM组(P<0.05),CD3、CD8+T细胞、CD4+CD25+T细胞低于单纯T2DM组。结论机体细胞免疫异常在T2DM及大血管病变的发生、发展中发挥着一定的作用。

Objective To investigate the changes of T lymphocyte subsets and their roles in the development of type 2 Diabetes（T2DM） and its macrovascular complications. Methods T lymphocyte subsets of periphera1 blood mononuclear cells（PBMCS） were determined with immunofluorescence staining and flow cytometry method in 36 patients with T2DM mellitus and 28 patients with macrovascular disease and 20 healthy subjects as the controls. Results The percentage of CD4^＋T cells,CD4/CD8,CD4^＋CD28^-T cells was increased while CD3＋T cells,CD8＋T cells,CD4^＋CD25^＋T cells were decreased in those with T2DM and macrovascular disease（P〈0.05）. There was significant difference between all of the above changes and those in the control group（P〈0.05）. CD4^＋T cells,CD4/CD8,CD4^＋CD28^-T cells in the group of macrovascular disease were also higher than those in T2DM and CD3,CD8＋T cells,CD4^＋CD25^＋ T cells were lower. Conclusion Immune dysfunction exert certain effects on the pathogenesis and progression of T2DM mellitus and macrovascular disease.