三聚氰胺和三聚氰酸混合物所致肾结石的实验研究

Empirical study of melamine and cyanuric acid leading renal calculus and therapeutic turnover.

目的评价枸橼酸和金钱草治疗三聚氰胺-三聚氰酸所致大鼠肾脏结石和急性肾功能损害的实验效果。方法40只sD幼龄鼠被随机分成4组。正常对照组（NC组）10只，常规喂养。三聚氰胺-三聚氰酸加自来水组（Mc组）、三聚氰胺-三聚氰酸加枸橼酸治疗组（CA组）、三聚氰胺-三聚氰酸加金钱草治疗组（HL组）各10只，三聚氰胺三聚氰酸悬浮液每日400／400mg／kg连续灌胃3d后，分别用自来水2mL／d、枸橼酸溶液每日1g／kg、金钱草溶液每日1g／kg连续灌胃10d。评价各组大鼠的平均体重、平均双侧肾重、肾重／体重、肾功能、肾脏组织病理学结果。结果MC组、CA组和HL组在三聚氰胺-三聚氰酸喂养期间平均体重进行性下降；停止喂养后分别用自来水、枸橼酸和金钱草溶液灌胃处理，平均体重逐渐上升。处理结束后3组平均体重显著低于NC组，平均双侧肾重、肾重／体重均明显高于NC组，肾功能明显受损，肾脏中均存在大量晶体。但MC组、CA组和HL组三组间平均体重、平均双侧肾重、肾重／体重、肾功能比较无统计学意义。结论三聚氰胺-三聚氰酸在大鼠肾脏中产生不溶性晶体并导致急性肾功能损害；短期的枸橼酸和金钱草治疗不能促进晶体排出或溶解晶体，不能改善肾功能。

Objective This paper describes research relating to the effects of Herba Lysimachiae and Citric Acid eliminated melamine-cyanuric acid calculus in rat kidneys. Methods 40 SD young rats were random divided into four groups： normal control （NC）, melamine-cyanuric acid （MC）, melamine-cyanuric acid and Citric Acid treatment group （CA）, as well as melamine-cyanuric acid and Herha Lysimachiae treatment group （HL）, and 10 rats for each group. The NC group was normal fed. For others group, the suspension of melamine-cyanuric acid were given by oral garage at a dosage of 400 / 400 mg/ kg / day for the first three consecutive days; and then, MC and CA and HL, tap water and Citric Acid and Herba Lysimachiae solution were respectively givern by oral gavage at 2 ml / day and lg / kg / day and lg / kg / day for the following second 10 consecutive days. Analyses included a range of indicators of renal damage, mean body weight, mean hibateral renal weight, renal weight / body weight, renal histopathology. Results In the MC, CA and HL groups, mean body weights were decreased during the three days melamine-cyanuric acid feeding, and were increased during ten days tap water or Citric Acid or Herba Lysimaehiae treatment. The three groups mean body weights were significantly lower than that in the NC group, and mean hibateral renal weights and renal weight / body weight were significantly higher than those in NC, renal function and histopathology did not ameliorated. But, there were not significant difference between the three groups. Melamine-cyanuric acid formed an insoluble crystals in renal tubules leading to acute renal function damage. Conclusions Melamine-cyanuric acid caused insoluble ci）,stals in rat kidneys and acute renal function damage. Neither Herba Lysimachiae nor Citric Acid produced any protective effect in short period.