异时相Cyclin B1/CDK1表达有助于正常在体细胞进入细胞分裂周期

The Unscheduled Expression of Cyclin B1/CDK1 Contributes to the Transition of Cell Cycle of Lymphocytes in vivo from G_0 to G_1 Phase

目的研究通常表达于G2/M期的Cyclin B1在G1期异时相表达的作用机制及意义。方法用丝裂原刺激因子(PHA)刺激人外周血淋巴细胞使之进入细胞周期,分别在刺激后0、36、48、60 h收集细胞,并分成两部分,一部分用流式Cyclins/DNA多参数技术分析,一部分用Post-sorting Western blot分析。结果处于G0期的淋巴细胞经PHA刺激进入细胞周期后,可以观察到Cyclin B1在G1期有异时相表达,相应的CDK1亦有异时相表达。结论异时相Cyc-lin B1/CDK1可能有助于正常在体细胞进入细胞分裂周期。

Objective To study the mechanism of unscheduled Cyclin B1 expression at G1 phase which is usually at G2/M phase.Methods Human peripheral blood lymphocytes（PBL）from healthy volunteers were firstly activated by PHA and then went into cell cycle.The cells were collected at 0,36,48 and 60 h after activation and divided into two parts：one for Cyclins/DNA multiparameter assay,and another for Post-sorting Western blot.Results After activation by PHA,Cyclin B1 and CDK1 of lymphocytes were expressed at G1 phase.Conclusion Unscheduled Cyclin B1/CDK1 probably contributes to lymphocytes in vivo into cell cycle.