“四天穴”针方对颈椎病模型大鼠外周炎性因子及基质金属蛋白酶表达的影响

Effect of sitian acupoint prescription on periphery inflammatory factor and matrix metalloproteinase expression in rats with disease of vertebrae cervicales

[目的]通过观察"四天穴"针方对动静力失衡性颈椎病模型大鼠椎间盘外周炎性因子白细胞介素-1β(IL-1β)、一氧化氮合酶(iNOS)、环氧酶-2(COX-2)及中性蛋白多糖酶(MMP-3)表达的影响,探讨其对颈椎病大鼠椎间盘退变的作用机制。[方法]Wistar大鼠60只,随机分为4组,即假手术组、模型组、四天穴针刺组、夹脊穴针刺组,建立动静力失衡性颈椎病模型,分别给予四天穴及夹脊穴针刺,浓缩型免疫组化染色法(SABC法)检测"四天穴"针方对动静力失衡性颈椎病模型大鼠造模后1周、2周、4周颈椎间盘IL-1β、iNOS、COX-2及MMP-3蛋白表达的影响。[结果]"四天穴"针方能明显抑制椎间盘炎性因子IL-1β、iNOS、COX-2及基质金属蛋白酶MMP-3蛋白的表达。[结论]"四天穴"针方可减缓颈椎间盘退变,其机制可能与抑制外周炎性因子与基质金属蛋白酶表达有关。

[Objective] To discuss the effective mechanism of Sitian acupoints prescription in treating disease of vertebrae cervicales of rats through observing IL-1β,iNOS,COX-2 and MMP-3 expression change. [Methods] Sixty Wistar rats were randomly divided into four groups：sham operated group,model group,Sitian acupoint acupuncture group,Jiaji acupoint group. The disease of vertebrae cervicales induced by imbalance of dynamic-static force was established. Treating with Sitian acupoint and jiaji acupoint was given for 4 days. IL-1,iNOS,COX-2 MMP-3 enzyme and MMP-3 protein were determined by SABC method. 1,2 and 4 weeks after disease. [Results] Sitian acupoint prescription could obviously inhibit IL-1β,iNOS,COX-2 and MMP-3 protein expression. [Conclusion] Sitian acupoint prescription may retard the retrograde change of cervical intervertebral disk and its effective mecha-nism might be related with inhibiting the periphery inflammatory factor and MMP-3 protein expression.