百合花粉及花粉管内微丝和微管的分布

DISTRIBUTION OF F ACTIN AND MICROTUBULES IN POLLEN AND POLLEN TUBE OF LILIUM DAVIDII 

利用免疫荧光定位及荧光定位方法，结合共焦激光扫描显微镜，对百合（ＬｉｌｉｕｍｄａｖｉｄｉＤｕｃｈ．）花粉及花粉管内微丝及微管的分布进行了观察，得出了一些新的结果：１化学固定方法可以较好地保存花粉和花粉管内的微丝，从而可以在此条件下较好地进行微管和微丝的双标记，并进行两者相互关系的研究；２在距花粉管顶端１０～２０μｍ的范围内，用化学固定及ＴＲＩＴＣ鬼笔碱标记显示微丝的存在是很微弱的，基本上无法看到明显的微丝束，而同时用免疫荧光法标记却发现此部位微管很丰富，在花粉管顶端微管形成浓密的网状，而且其末端与花粉管顶端质膜相连；３在花粉管中，只有少数微丝与微管相互平行排列，而其中大多数微丝骨架与微管骨架并不存在共分布现象。为了解花粉管内微管和微丝的功能及相互关系提供了新的证据。

F actin and microtubules are important components of pollen tube, which have very important function in cytoplasm streaming of pollen tube. The authors observed the distribution of F actin and microtubules in the pollen tube of Lilium davidii Duch. by immunofluorescence technique and confocol laser scanning microscopy, through which some new results were obtained. 1. Chemical fixation could preserve F actin well in pollen tube, so the relation between F actin and microtubules could be studied by the methods of chemical fixation and fluorescence labelling in pollen tube. 2. F actin bundles were absent near the pollen tube tip, while microtubules were abundant and web formed in the pollen tube tip. The authors found that the terminal of microtubules was closely associated with the plasma membrane in the pollen tube tip. 3. Only a few F actin bundles co exist with the microtubules in the pollen tube of Lilium davidii . The results provided new evidence for the function and relationship between F actin and microtubules in the pollen tube.