摘要
通过Ｗｅｓｔｅｒｎ印渍方法，发现在豌豆（ＰｉｓｕｍｓａｔｉｖｕｍＬ．）叶存在一种高分子量的蛋白可与ＧｒｏＥＬ抗体发生免疫学反应，它含有两种亚基，分子量分别为６０．４ｋＤ（α）和６５．５ｋＤ（β），具有弱的ＡＴＰａｓｅ活性。热激处理后此蛋白的表达升高３～４倍，但外源ＡＢＡ处理并不增加它的表达。用３５Ｓ标记蛋白和氯霉素抑制实验表明，此蛋白可能是在８０Ｓ核糖体上合成的胞质蛋白。
A high molecular weight protein was found in pea (Pisum sativum L.) seedling by means of Western blotting, and it consisted of a α (60.4 kD) and a β (65.5 kD) subunits. The protein had low ATPase activity. Its expression could be enhanced by 3 to 4 folds under heat shock stress, but was not affected by exogenous ABA. The results of localization and 35 S Met labelling showed that it was a cytoplasmic protein and its synthesis was not inhibited by chloramphenicol.
基金
国家自然科学基金重点项目
