摘要
应用整装电镜制样技术，结合选择性抽提方法在铁线蕨（ＡｄｉａｎｔｕｍｐｈｉｌｉｐｐｅｎｓｅＬ．）叶细胞中观察到直径１０ｎｍ的纤维网络结构。免疫印迹结果显示：经选择性抽提得到的纤维蛋白与动物角蛋白抗体有免疫交叉反应，间接免疫荧光标记也得到类似结果，而且此类蛋白能在体外自组装为１０ｎｍ或更粗的纤维。说明蕨类植物细胞中存在类角蛋白中间纤维网络。免疫印迹结果表明，螺旋藻（ＳｐｉｒｕｌｉｎａｓｕｂｔｉｌｌｉｓｉｎａＫｕｔｚ．）细胞，地钱（ＭａｒｃｈａｎｔｉａｐｏｌｙｍｏｒｐｈａＬ．）叶状体，铁线蕨（Ａ．ｐｈｉｌｉｐｐｅｎｓｅＬ．）、银杏（ＧｉｎｋｇｏｂｉｌｏｂａＬ．）、白菜（ＢｒａｓｉｃａｐｅｋｉｎｅｎｓｉｓＲｕｐｒ．）的叶组织经选择性抽提后得到的蛋白均与动物角蛋白抗体有免疫交叉反应。其中，螺旋藻仅含有两种类酸性角蛋白，而其余４种植物材料均含有３种类酸性角蛋白及３种类碱性角蛋白。结合以往实验结果，我们认为类角蛋白在植物细胞中是普遍存在的。
Network of filaments, 10 nm in diameter, was detected in the frond cells of Adiantum philippense L. by selective extraction combined with whole mount electron microscopy. Western blot analysis showed that the major filament components were cross reacted with monoclonal antibodies against animal keratin. The phenomenon was in concert with the result of indirect immunofluorescence observation. The filaments with 10 nm in diameter could be reassembled in vitro . Using selective extraction, proteins were prepared from cells of Spirulina subtillissima Kutz., thalli of Marchantia polymorpha L., and leaf blades of A. philippense L., Ginkgo biloba L. and Brassica pekinensis Rupr. Western blot showed that S. subtillissima Kutz. has two acidic keratin like proteins and all of the other four plant species have three basic keratin like proteins and three acidic keratin like proteins. These results may suggest the existance of an intermediate filament network with keratin like proteins in the cells of pteridophyte and their existance in plant cells is popular.
关键词
铁线蕨
植物细胞
中间纤维
类角蛋白
选择性抽提
Adiantum philippense, Plant cell, Intermediate filament, Keratin like protein, Selective extraction, Western blot
