中国不同来源狂犬病病毒野毒株G基因主要功能区的序列分析

SEQUENCING OF GLYCOPROTEIN ECTODOMAIN GENE OF RABIES STREET VIRUSES FROM DIFFERENT ANIMALS IN CHINA

对我国不同来源的狂犬病病毒野毒株８２０２、ＢＲＶ、ＭＲＶ的Ｇ基因４０５～１１４６位核苷酸序列，进行了逆转录－聚合酶链反应（ＲＴ－ＰＣＲ）扩增、克隆和序列测定，并应用计算机对这３个毒株的测定序列和已发表的中国人源毒株（ＣＧＸ８９）的相应序列进行了分析比较。结果表明，这４个中国不同来源狂犬病病毒的Ｇ基因同源性较低，８２０２与ＢＲＶ的核苷酸同源性只有７９．５％，与ＭＲＶ的氨基酸同源性亦只有８２．２％；同一地区自不同动物分离的ＭＲＶ株与ＢＲＶ株其亲缘关系较远。４个野毒株的糖基化位点和主要的诱导中和抗体产生的抗原决定簇内某些氨基酸亦不同，从而证明我国存在亲缘关系较远的狂犬病野毒株。

The fragment from 405nt to 1146 nt of glycoprotein(G) ectodomain gene of rabies virus strains 8202,BRV and MRV,isolated from deer,calf,mouse respectively in various areas of China,were amplified by RT-PCR.Then they were cloned and sequenced by Sanger's dideoxy method.Comparison of nucleotide and deduced amino acid sequences of them and the previously published CGX89 strain (from human) was performed by computer with DNASIS software.The results showed that homology of the nucleotide sequences and the amino acid sequences of four Chinese rabies viruses were very low.Homology of the nucleotide sequence of 8202 with BRV was only 79.5% and homology of amino acid sequence of 8202 with MRV was 82.2%.The fact that homology of BRV with MRV,isolated from different animals in Henan area,was only 81.1% showed that they could not be from the same ancestor.Some amino acids in Ag Ⅱ and Ag Ⅲ sites and potential glycosylation sites of four strains were different.This result proved that rabies viruses from China might be divided into different genotypes.