氟化物对幼年大鼠骨胶原的影响

STUDY OF FLUORIDES ON THE COLLAGEN IN RATS IN GROWTH PERIOD

目的探讨氟化钠对幼年大鼠骨胶原影响。方法90只2月龄SPF级SD大鼠,雌雄各半,随机分成9组:对照(幼年CS、成年AS、长期NS)组和用药组(幼年高氟组CHS、幼年低氟组CLS、成年高氟组AHS、成年低氟组ALS、幼年长期高氟组CHS、幼年长期低氟组CLS)。对照组灌胃生理盐水,用药组分别按相应时间给予不同剂量的NaF灌胃。实验验结束后,大鼠被异戊巴比妥钠麻醉后,心脏取血处死大鼠。检测血清中PICP和ICTP的含量。左侧尺骨用盐酸溶解后测定其骨羟脯氨酸(Hyp)的含量。结果①与CS组相比,CHS组和CLS组的PICP则分别增加了38.1%和38.4%(p<0.05),而ICTP的变化无统计学意义;CHS组和CLS组的Hyp分别下降了45.7%和38.6%(p<0.05)。②与NS组相比,HS组的PICP下降了30.2%(p<0.05)。③与HS组相比,LS组的PICP比HS组高64.86%(p<0.05),LS组的Hyp下降了26.6%(p<0.05),而LS组的ICTP的变化无统计学意义。与HS组相比,CHS组的PICP增高了高100%,(p<0.05),而ICTP的变化无统计学意义。④与NS组相比,HS组的HYP下降47.3%(p<0.05),LS组HYP变化均无显著性意义。结论长期使用氟化钠,可以抑制幼年大鼠的PICP合成,导致骨胶原丢失。

Objective To study the effect of sodium fluoride on the collagen in rats in growth period. Methods Ninety 2 - month - old SPF Sparague - Dawley rats, half male and female, were randomly divided into 9 groups ： control （ childhood, adult） group and drug - administered group （ childhood, adult high - fluoride and low - fluoride group, long - term high - fluoride and low - fluoride group）. The control group were administered orally with solution of 0. 9% NaCl, while the drug delivery group were given orally with different dose NaF by the corresponding time. At the experimental endpoint, rats were sacrificed by cardiac puncture under sodium pontobarbital anesthesia. The blood plasma was used to test the content of PICP and /CTP. The serum was applied to test the contents of hydroxyproline. Results （1）Compared with CS group, the PICP of the CHS group and CLS group was increased by 38. 1% and 38.4% （p 〈0.05） respectively. The change of ICTP of the CHS group and CLS group had not meet statistical significance. The Hyp of the CHS group and CLS group was decreased by 45.7% and 38.6% （p 〈0. 05） respectively. （2）Compared vdth NS group, the PICP of HS group was losed by30. 2%. （3）Compared with HS group, the PICP of the LS group was added 64. 86% ,but the Hyp of the LS group was dropped 26.6% （p 〈0.05）. The ICTP in LS group had no obvious change. Compared with HS group, the PICP of CHS group were added by 100% （p 〈0. 05） , and the ICTP in LS group had no obvious change,too. （4）Compared with NS group, the Hyp of HS group was losed by 47. 3% （p 〈0. 05）, and the Hyp in LS group had no obvious change. Conclusion The long -term use of sodium fluoride can lead to inhibit the synthesis of PICP in rats in growth period, resulting in the loss of bone collagen in rats in growth period.